- 28-8-6 (See other available formats)
- Regulatory Status
- Other Names
- MHC class I
- Mouse (C3H) IgG2a, κ
- Ave. Rating
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- Product Citations
The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.Product Details
- Mouse b haplotype, Cross-reacts with H-2Dd MHC class I alloantigen
- Antibody Type
- Host Species
- C3H.SW mouse splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
- Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
- Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
- Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
- Product Citations
AB_2750199 (BioLegend Cat. No. 114619)
AB_2750200 (BioLegend Cat. No. 114620)
- MHC class I
Nucleated cells of the H-2Kb/H-2Db haplotype
- Antigen presentation
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.
- Gene ID
- 14964 View all products for this Gene ID 14972 View all products for this Gene ID
- View information about H-2K b,H-2D b on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|FITC anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|Purified anti-mouse H-2Kb/H-2Db||28-8-6||FC, IHC-F|
|Biotin anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|PE anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|APC/Fire™ 750 anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|APC anti-mouse H-2Kb/H-2Db||28-8-6||FC|
|PE/Cyanine7 anti-mouse H-2Kb/H-2Db||28-8-6||FC|
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