| Fluorophore | Ex (nm) Max | Em (nm) Max | Filter Used | Brightness |
|---|---|---|---|---|
| PE | 565 | 575 | 585/20 | 5 |
| PE/Cyanine5 | 565 | 670 | 660/20 | 5 |
| PE/Dazzle™ 594 | 565 | 610 | 610/20 | 5 |
| PE/Fire™ 700* | 565 | 695 | - | 5 |
| Alexa Fluor® 647 | 650 | 668 | 660/20 | 4 |
| APC | 650 | 660 | 660/20 | 4 |
| Brilliant Violet 421™ | 405 | 421 | 450/50 | 4 |
| Brilliant Violet 711™ | 405 | 711 | 710/50 | 4 |
| PE/Cyanine7 | 565 | 774 | 780/60 | 4 |
| PE/Fire™ 640* | 565 | 639 | - | 4 |
| PE/Fire™ 810* | 565 | 806 | - | 4 |
| APC/Fire™ 810* | 650 | 807 | - | 3 |
| Brilliant Violet 605™ | 405 | 603 | 610/20 | 3 |
| Brilliant Violet 650™ | 405 | 645 | 660/20 | 3 |
| Brilliant Violet 750™ | 405 | 750 | 780/60 | 3 |
| Brilliant Violet 785™ | 405 | 785 | 780/60 | 3 |
| Spark YG™ 593* | 574 | 593 | - | 3 |
| KIRAVIA Blue 520™ | 488 | 520 | 530/30 | 3 |
| Alexa Fluor® 700 | 696 | 719 | 720/45 | 2 |
| APC/Cyanine7 | 650 | 774 | 780/60 | 2 |
| APC/Fire™ 750 | 650 | 774 | 780/60 | 2 |
| PerCP/Cyanine5.5 | 482 | 690 | 695/40 | 2 |
| Spark NIR™ 685* | 660 | 685 | - | 2 |
| Spark YG™ 581* | 565 | 581 | - | 2 |
| Alexa Fluor® 488 | 495 | 519 | 530/30 | 1 |
| Brilliant Violet 510™ | 405 | 510 | 510/50 | 1 |
| Brilliant Violet 570™ | 405 | 570 | 585/42 | 1 |
| FITC | 493 | 525 | 530/30 | 1 |
| Pacific Blue™ | 410 | 455 | 450/50 | 1 |
| PerCP | 482 | 675 | 695/40 | 1 |
| Spark Blue™ 550* | 516 | 540 | - | 1 |
| Spark Violet™ 538 | 399 | 538 | - | 1 |
*Dyes denoted by an asterisk are recommended to be used on spectral unmixing flow cytometers. They may be compatible with conventional flow cytometers, but end users may need to optimize filter sets.
