APC anti-mouse Perforin Antibody

Pricing & Availability
S16009A (See other available formats)
Regulatory Status
Other Names
Prf1, Pfp, Pfn, Pfn-1
Rat IgG2a, κ
Ave. Rating
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Product Citations
C57BL/6 splenocytes were stained with CD49b PE. Cells were fixed, permeabilized, and stained with APC anti-mouse Perforin (clone S16009A, top) or APC rat IgG2a, κ isotype control (bottom).
  • S16009A_APC_Perforin_Antibody_1_111617
    C57BL/6 splenocytes were stained with CD49b PE. Cells were fixed, permeabilized, and stained with APC anti-mouse Perforin (clone S16009A, top) or APC rat IgG2a, κ isotype control (bottom).
  • S16009A_APC_Perforin_Antibody_2_111617
Compare all formats See APC spectral data
Cat # Size Price Save
154303 25 µg ¥28,980
154304 100 µg ¥72,870

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse perforin transfected cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.

Product Citations
  1. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  2. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  3. Haque A, et al. 2020. Exp Neurol. 330:113315. PubMed
AB_2721462 (BioLegend Cat. No. 154303)
AB_2721463 (BioLegend Cat. No. 154304)

Antigen Details

70 kD Protein that oligomerizes for pore formation.

CTL, NK (Cytoplasmic Granules)

Target cell lysis
Cell Type
NK cells, T cells
Biology Area
Molecular Family
Antigen References
  1. Podack ER, et al. 1988. Immunol. Rev. 103:203.
  2. Konjar S, et al. 2010. Immunology. 131:257.
  3. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
  4. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID
18646 View all products for this Gene ID
View information about Perforin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 11/16/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Pacific Blue™ anti-mouse Perforin

  • Alexa Fluor® 647 anti-mouse Perforin


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