Alexa Fluor® 700 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_Alx700_021808
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
  • 17A2_Alx700_021808
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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100215 25 µg 76€
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100216 100 µg 172€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zaitseva M, et al. 2017. Antiviral Research. 10.1016/j.antiviral.2017.05.002. PubMed
  2. Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed
  3. Watanabe K, et al. 2018. JCI Insight. 3. PubMed
  4. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  5. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  6. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  7. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  8. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  9. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  10. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  11. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  12. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
  13. Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed
  14. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  15. Agashe VV, et al. 2018. J Immunol. 201:772. PubMed
  16. Li Q, et al. 2019. Front Pharmacol. 10:786. PubMed
  17. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  18. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  19. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  20. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  21. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  22. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  23. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  24. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  25. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  26. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  27. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  28. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  29. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  30. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  31. Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed
  32. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  33. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  34. Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed
  35. Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed
  36. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  37. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  38. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  39. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  40. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  41. Wojno E, et al. 2011. J Immunol. 187:266. PubMed
  42. Abdul-Careem MF, et al. 2012. PLoS One. 7:e32821. PubMed
  43. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  44. Mykkänen O, et al. 2014. PLoS One. 9:114790. PubMed
  45. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  46. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  47. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  48. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  49. Li A, et al. 2020. J Virol. 94:00:00. PubMed
  50. Tyagi AM, et al. 2021. eLife. 10:00. PubMed
  51. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  52. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
RRID
AB_493696 (BioLegend Cat. No. 100215)
AB_493697 (BioLegend Cat. No. 100216)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC, ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F, FC, 3D IHC, SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

  • Spark Red™ 718 anti-mouse CD3

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