- 4E5 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily. Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J. The receptors form homodimers that bind to MHC class I alloantigens. Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif. The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens. Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells. In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.
(PubMed link indicates BioLegend citation)
- Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
- Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
- George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
- Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
- Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
- Product Citations
AB_10588709 (BioLegend Cat. No. 138303)
- A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
- Plays an important role in activating natural killer (NK) cell cytolytic activity.
- MHC class I alloantigens
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.
- Gene ID
- 16635 View all products for this Gene ID
- View information about Ly49D on UniProt.org
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