- L5 (See other available formats)
- Regulatory Status
- Other Names
- FLAG tag
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
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The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.Product Details
- Verified Reactivity
- DYKDDDDK tag epitope
- Antibody Type
- Host Species
- DYKDDDDK-tagged mouse Langerin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
(PubMed link indicates BioLegend citation)
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
AB_2750051 (BioLegend Cat. No. 637321)
AB_2750061 (BioLegend Cat. No. 637322)
- Biology Area
- Cell Biology
- Antigen References
1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.
- Gene ID
- View information about DYKDDDDK on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
|Purified anti-DYKDDDDK Tag||L5||WB, IP, ICC, ELISA, FC, Purification|
|Anti-DYKDDDDK Tag (L5) Affinity Gel||L5||Purification, IP|
|APC anti-DYKDDDDK Tag||L5||FC, ICFC|
|PE anti-DYKDDDDK Tag||L5||FC, ICFC|
|Direct-Blot™ HRP anti-DYKDDDDK Tag||L5||WB|
|Alexa Fluor® 594 anti-DYKDDDDK Tag||L5||ICC|
|Alexa Fluor® 647 anti-DYKDDDDK Tag||L5||ICC|
|Alexa Fluor® 488 anti-DYKDDDDK Tag||L5||ICC|
|PE/Cyanine7 anti-DYKDDDDK Tag||L5||FC|
|Brilliant Violet 421™ anti-DYKDDDDK Tag||L5||FC|
|PerCP/Cyanine5.5 anti-DYKDDDDK Tag||L5||FC|
|Ultra-LEAF™ Purified anti-DYKDDDDK Tag||L5||WB, IP, ICC, ELISA, FC, Purification|
|PE/Dazzle™ 594 anti-DYKDDDDK Tag Antibody||L5||FC|
|TotalSeq™-B1129 anti-DYKDDDDK Tag||L5||PG|
|TotalSeq™-A1129 anti-DYKDDDDK Tag||L5||PG|
|TotalSeq™-C1129 anti-DYKDDDDK Tag||L5||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-DYKDDDDK Tag
Anti-DYKDDDDK Tag (L5) Affinity Gel
APC anti-DYKDDDDK Tag
PE anti-DYKDDDDK Tag
Direct-Blot™ HRP anti-DYKDDDDK Tag
Alexa Fluor® 594 anti-DYKDDDDK Tag
Alexa Fluor® 647 anti-DYKDDDDK Tag
Alexa Fluor® 488 anti-DYKDDDDK Tag
PE/Cyanine7 anti-DYKDDDDK Tag
Brilliant Violet 421™ anti-DYKDDDDK Tag
PerCP/Cyanine5.5 anti-DYKDDDDK Tag
Ultra-LEAF™ Purified anti-DYKDDDDK Tag
PE/Dazzle™ 594 anti-DYKDDDDK Tag Antibody
TotalSeq™-B1129 anti-DYKDDDDK Tag
TotalSeq™-A1129 anti-DYKDDDDK Tag
TotalSeq™-C1129 anti-DYKDDDDK Tag