APC anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_APC_031808
C57BL/6 mouse splenocytes stained with H57-597 APC
  • H57-597_APC_031808
    C57BL/6 mouse splenocytes stained with H57-597 APC
See APC spectral data
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109211 25 µg £48.00
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109212 100 µg £123.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Kanuma T, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1600731. PubMed
  2. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  3. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  4. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  5. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  6. Chen L, et al. 2018. Immunology. 155:123. PubMed
  7. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  8. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  9. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  10. Chuang YT, et al. 2019. J Allergy Clin Immunol. 143:565. PubMed
  11. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  12. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  13. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  14. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  15. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  16. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  17. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  18. Preece R, et al. 2020. Mol Ther Methods Clin Dev. 0.895138889. PubMed
  19. Andrews D, et al. 2008. J Virol. 82:4931. PubMed
  20. Ley S 2009. J Immunol. 183:865. PubMed
  21. Andrews D, et al. 2010. J Exp Med. 207:1333. PubMed
  22. Zhu M, et al. 2012. J Biol Chem. 287:8135. PubMed
  23. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  24. Mallis R, et al. 2015. Proc Natl Acad Sci U S A. 112: 8373 - 8378. PubMed
  25. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  26. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  27. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  28. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  29. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  30. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  31. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  32. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  33. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  34. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
RRID
AB_313434 (BioLegend Cat. No. 109211)
AB_313435 (BioLegend Cat. No. 109212)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/06/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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