PE anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_PE_083106
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) stained with H1.2F3 PE
  • H1dot2F3_PE_083106
    PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) stained with H1.2F3 PE
See PE spectral data
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104507 50 µg 80,00€
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104508 200 µg 200,00€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  2. Yan Q, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.05.006. PubMed
  3. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  4. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  5. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  6. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
  7. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  8. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  9. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  10. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  11. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  12. Skadow M, et al. 2019. J Immunol. 203:370. PubMed
  13. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  14. Amantini C, et al. 2017. Oncotarget. 8:90766. PubMed
  15. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  16. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  17. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  18. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  19. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  20. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  21. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
  22. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  23. Holan V, et al. 2010. J Immunol. 184:2124. PubMed
  24. Huang Z, et al. 2012. J Immunol. 188:5867. PubMed
  25. Yuan Z, et al. 2011. Vaccine. 29:6614. PubMed
  26. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  27. Passos C, et al. 2014. Food Chem. 15:161. PubMed
  28. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  29. Artinger K, et al. 2015. PLoS One. 10: 0135087. PubMed
  30. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  31. Jang A, et al. 2015. Life Sci . 135: 138-146. PubMed
  32. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  33. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  34. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  35. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  36. Velázquez K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G699 - G712. PubMed
  37. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  38. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  39. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  40. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  41. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  42. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  43. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  44. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  45. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  46. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  47. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  48. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  49. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  50. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  51. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  52. Hu J, et al. 2020. Biomed Pharmacother. 127:110229. PubMed
RRID
AB_313110 (BioLegend Cat. No. 104507)
AB_313111 (BioLegend Cat. No. 104508)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 04/18/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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