- HI30 (See other available formats)
- Regulatory Status
- IV N816
- Other Names
- LCA, T200
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.Product Details
- Human, Cross-Reactivity: Chimpanzee
- Antibody Type
- Host Species
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, and Western blotting3.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
- Application References
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K, et al. 1993. Cell 74:143.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
- Nagano M, et al. 2007. Blood 110:151.
- Jiang Q, et al. 2008. Blood 112:2858. PubMed
- Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Product Citations
AB_314395 (BioLegend Cat. No. 304007)
AB_314396 (BioLegend Cat. No. 304008)
AB_2564156 (BioLegend Cat. No. 304058)
AB_2562057 (BioLegend Cat. No. 304039)
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- TCR and BCR mediated activation
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- View information about CD45 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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