Pacific Blue™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HCD56_PB_060710
Human peripheral blood lymphocytes stained with HCD56 Pacific Blue™
  • HCD56_PB_060710
    Human peripheral blood lymphocytes stained with HCD56 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
318325 25 µg 93€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
318326 100 µg 199€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  2. Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed
  3. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  4. Goodridge J, et al. 2013. J Immunol. 191:3553. PubMed
  5. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  6. Jeffery H, et al. 2017. Clin Exp Immunol. 10.1111/cei.12940. PubMed
  7. Roerden M, et al. 2021. Cancers. 13(11):. PubMed
RRID
AB_10612566 (BioLegend Cat. No. 318325)
AB_10612751 (BioLegend Cat. No. 318326)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD56 (NCAM)

  • FITC anti-human CD56 (NCAM)

  • PE anti-human CD56 (NCAM)

  • PE/Cyanine5 anti-human CD56 (NCAM)

  • APC anti-human CD56 (NCAM)

  • Alexa Fluor® 488 anti-human CD56 (NCAM)

  • Alexa Fluor® 647 anti-human CD56 (NCAM)

  • Alexa Fluor® 700 anti-human CD56 (NCAM)

  • PE/Cyanine7 anti-human CD56 (NCAM)

  • Biotin anti-human CD56 (NCAM)

  • PerCP/Cyanine5.5 anti-human CD56 (NCAM)

  • Pacific Blue™ anti-human CD56 (NCAM)

  • APC/Cyanine7 anti-human CD56 (NCAM)

  • Brilliant Violet 421™ anti-human CD56 (NCAM)

  • Brilliant Violet 570™ anti-human CD56 (NCAM)

  • Brilliant Violet 605™ anti-human CD56 (NCAM)

  • Brilliant Violet 711™ anti-human CD56 (NCAM)

  • Brilliant Violet 510™ anti-human CD56 (NCAM)

  • PerCP anti-human CD56 (NCAM)

  • Brilliant Violet 650™ anti-human CD56 (NCAM)

  • Purified anti-human CD56 (NCAM) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD56 (NCAM)

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account