APC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5slash114_APC_011108
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • M5slash114_APC_011108
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
107613 25 µg 48,00€
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107614 100 µg 152,00€
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
  2. Xu X, et al. 2018. Cell. 175:1336. PubMed
  3. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  4. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  5. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  6. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  7. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  8. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  9. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  10. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  11. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  12. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  13. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  14. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  15. Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed
  16. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  17. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  18. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  19. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  20. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  21. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  22. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
  23. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  24. Gray D, et al. 2007. J Exp Med. 204:2521. PubMed
  25. Rayamajhi M, et al. 2010. J Exp Med. 207:327. PubMed
  26. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  27. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  28. Kitur K, et al. 2015. PLoS Pathog. 11:1004820. PubMed
  29. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  30. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  31. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  32. Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed
  33. Boucard-Jourdin M, et al. 2016. J Immunol. 197: 1968 - 1978. PubMed
  34. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  35. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  36. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  37. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  38. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  39. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  40. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  41. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
RRID
AB_313328 (BioLegend Cat. No. 107613)
AB_313329 (BioLegend Cat. No. 107614)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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