Purified anti-human CD64 Maxpar Ready Antibody anti-CD64

Pricing & Availability

Clone: 10.1 (See other available formats)
Regulatory Status: RUO
Workshop: VI MA36
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ
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Product Citations: publications

10.1_Purified_CD64_Cytof_060514.jpg — Human PBMCs stained with 158Gd-anti-CD33 (WM53) and 146Nd-anti-CD64 (10.1). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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305029

100 µg

121 CHF

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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Holl V, et al. 2004. J. Immunol. 173:6274.
Hober D, et al. 2002. J. Gen. Virol. 83:2169.
Cho HJ, et al. 2007. Physiol Genomics 149:60.
van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
Bruhns P, et al. 2008. Blood 113:3716. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Carter DL, et al. 1999. Cytometry 37:41. (FC)
Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)

Product Citations

Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed

RRID

AB_2563759 (BioLegend Cat. No. 305029)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD64 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
Purified anti-human CD64	10.1	FC, IHC-F, Block
Alexa Fluor® 488 anti-human CD64	10.1	FC
Alexa Fluor® 647 anti-human CD64	10.1	FC
APC anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
Brilliant Violet 421™ anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
APC/Cyanine7 anti-human CD64	10.1	FC
Brilliant Violet 510™ anti-human CD64	10.1	FC
Purified anti-human CD64 (Maxpar® Ready)	10.1	FC, CyTOF®
PE/Dazzle™ 594 anti-human CD64	10.1	FC
Brilliant Violet 605™ anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
PE/Dazzle™ 594 anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
TotalSeq™-A0162 anti-human CD64	10.1	PG
Brilliant Violet 711™ anti-human CD64	10.1	FC
Alexa Fluor® 700 anti-human CD64	10.1	FC
Brilliant Violet 785™ anti-human CD64	10.1	FC
TotalSeq™-C0162 anti-human CD64	10.1	PG
Ultra-LEAF™ Purified anti-human CD64	10.1	FC, IHC-F, Block
TotalSeq™-B0162 anti-human CD64	10.1	PG
TotalSeq™-D0162 anti-human CD64	10.1	PG
GMP PE anti-human CD64	10.1	FC
GMP FITC anti-human CD64	10.1	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07.22.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.