Alexa Fluor® 700 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_AF700_072210
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
  • 1A8_AF700_072210
    C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
127621 25 µg 105,00 CHF
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127622 100 µg 225,00 CHF
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Toomey C, et al. 2015. Proc Natl Acad Sci U S A. 112:3040. PubMed
  2. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  3. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  4. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
  5. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  6. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  7. Lin J, et al. 2017. Sci Rep. 7:41722. PubMed
  8. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  9. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  10. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  11. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  12. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  13. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  14. Dong L, et al. 2021. Cancer Cell. . PubMed
  15. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  16. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  17. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  18. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  19. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  20. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  21. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  22. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  23. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  24. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  25. Salas–Pérdomo A, et al. 2019. Sci Rep. 9:8309. PubMed
  26. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  27. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  28. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  29. Nalbandian A, et al. 2017. Inflammation. 40:21:00. PubMed
  30. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  31. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  32. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  33. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  34. Da Silva CG, et al. 2019. Theranostics. 4.878472222. PubMed
  35. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  36. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  37. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  38. Jin C, et al. 2019. Cell. 176:998. PubMed
  39. Flak MB, et al. 2020. J Clin Invest. 130:359. PubMed
RRID
AB_10640452 (BioLegend Cat. No. 127621)
AB_10643269 (BioLegend Cat. No. 127622)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01.06.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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