PE anti-mouse IL-31 Antibody

Pricing & Availability
Clone
W17037B (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-31
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17037B_PE_IL-31_Antibody_070920.png
PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized BALB/c mouse CD4 T cells were stained with CD3 FITC. After fixation and permeablization, cells were stained intracellularly with anti-mouse IL-31 PE (clone W17037B) (left) or rat IgG2a, κ PE isotype control (right).
  • W17037B_PE_IL-31_Antibody_070920.png
    PMA+ionomycin-stimulated (6 hours, in presence of brefeldin A) Th2-polarized BALB/c mouse CD4 T cells were stained with CD3 FITC. After fixation and permeablization, cells were stained intracellularly with anti-mouse IL-31 PE (clone W17037B) (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
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160703 25 µg $150.00
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160704 100 µg $280.00
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Description

IL-31, mainly produced by activated CD4+ T cells, is a helical cytokine belonging to the gp130/IL-6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune response. Transgenic mice overexpressing IL-31 developed severe dermatitis, indicating that IL-31 is involved in skin immunity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse IL-31
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876573 (BioLegend Cat. No. 160703)
AB_2876573 (BioLegend Cat. No. 160704)

Antigen Details

Structure
Member of IL-6 family of cytokines
Distribution

Subset of CD4 T cells

Ligand/Receptor
IL-31Ra/OSMR
Cell Type
Lymphocytes, T cells, Th2, Thymocytes
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
  2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
  3. Dai X, et al. 2013. PLoS One. 8:e67666.
  4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
  5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
  6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5
Gene ID
76399 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/09/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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