Brilliant Violet 421™ anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_BV421_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • BM8_BV421_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • BM8_BV421_F4slash80_Antibody_2_IHCF_060616
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 2 µg/ml of anti-mouse F4/80 (clone BM8) Brilliant Violet 421™ (Blue) and anti-mouse CD8a (clone 53-6.7) Alexa Fluor® 647 (red) overnight at 4°C. The image was captured with a 10X objective.
See Brilliant Violet 421™ spectral data See high resolution IHC data...
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123131 125 µL $185.00
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123137 50 µg $230.00
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123132 500 µL $360.00
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Glass A, et al. 2013. J Immunol. 190:4830. PubMed
  2. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  3. Textor A, et al. 2014. Cancer Res. 74:6769. PubMed
  4. Karsten C, et al. 2015. J Immunol. 194:1841. PubMed
  5. Müller P, et al. 2015. Sci Transl Med. 7: 315ra188. PubMed
  6. Zahr A, et al. 2016. Nat Commun. 7:10363. PubMed
  7. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  8. Crichton M, et al. 2016. Sci Rep. 6:27217. PubMed
  9. Sun K, et al. 2016. J Exp Med. 213: 1851 - 1864. PubMed
  10. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  11. Suzuki T, et al. 2017. Cell Rep. 18(8):2045-2057. PubMed
  12. Li J, et al. 2020. Elife. 9:00. PubMed
  13. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  14. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  15. Jaiswal AK, et al. 2020. Immunobiology. 225:151956. PubMed
  16. Cao DY, et al. 2020. J Biol Chem. 295:1369. PubMed
  17. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  18. Wang Y, et al. 2021. Nat Commun. 0.570833333. PubMed
  19. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  20. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  21. Shan Z, et al. 2021. eLife. 10:00. PubMed
  22. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  23. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  24. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  25. Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed
  26. Iturri L, et al. 2021. Immunity. . PubMed
  27. Yao W, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.07.014. PubMed
  28. Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed
  29. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  30. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  31. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  32. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  33. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  34. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  35. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  36. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  37. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  38. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  39. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  40. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  41. He J, et al. 2019. J Clin Invest. 130. PubMed
  42. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  43. Lim J et al. 2019. Elife. 8 pii: e44452. PubMed
  44. Kobayashi S, et al. 2019. J Immunol. 203:1447. PubMed
  45. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  46. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  47. Topper MJ et al. 2017. Cell. 171(6):1284-1300 . PubMed
  48. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  49. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  50. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  51. Brunner JS, et al. 2020. Nat Commun. 0.757638889. PubMed
  52. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  53. Lu Y, et al. 2020. Immunity. 52:782. PubMed
  54. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  55. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  56. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  57. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  58. McDonald LT, et al. 2018. Am J Physiol Heart Circ Physiol. 315:H92. PubMed
  59. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  60. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  61. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  62. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
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  64. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
RRID
AB_10901171 (BioLegend Cat. No. 123131)
AB_2563102 (BioLegend Cat. No. 123137)
AB_11203717 (BioLegend Cat. No. 123132)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
F4/80
Specificity Alt (DOES NOT SHOW ON TDS):
F4/80
UniProt
View information about F4/80 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 06/06/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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