APC anti-mouse VISTA (PD-1H) Antibody

Pricing & Availability
Clone
MIH63 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MIH63_APC_VISTA_Antibody_FC_1_092815
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MIH63) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • MIH63_APC_VISTA_Antibody_FC_1_092815
    C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MIH63) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • MIH63_APC_VISTA_Antibody_FC_2_092815
See APC spectral data
Cat # Size Price Quantity Check Availability Save
150205 25 µg $125.00
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150206 100 µg $305.00
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein that is composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and natural killer cells (NK). It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants due to its ability to preferentially suppress CD4+ T cell-mediated immunity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse VISTA transfected J558 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2566413 (BioLegend Cat. No. 150205)
AB_2566414 (BioLegend Cat. No. 150206)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family.
Distribution

Macrophages, dendritic cells, neutrophils, natural killer cells, and naive and activated T cells.

Function
Negative regulator of the immune response.
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp. Med. 208:577.
3. Flies DB, et al. 2014. J. Clin. Invest. 124:1966.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about VISTA on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/29/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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