- BCL/10C4 (See other available formats)
- Regulatory Status
- Other Names
- B-cell leukemia 2
- Mouse IgG1, κ
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- Product Citations
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Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- N-terminal, Amino acid residues 61-76 of mouse Bcl-2
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2064149 (BioLegend Cat. No. 633509)
AB_2274702 (BioLegend Cat. No. 633510)
- Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
- Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
- Phosphorylation, Ubiquitination
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
- Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
- Gene ID
- 596 View all products for this Gene ID
- View information about Bcl-2 on UniProt.org
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