PE/Cyanine7 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M1slash70_PECy7_042706
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram) (gated on total cells).
  • M1slash70_PECy7_042706
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram) (gated on total cells).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
101215 25 µg £55.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101216 100 µg £133.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  2. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  3. Gharib S, et al. 2014. J Leukoc Biol. 95:9. PubMed
  4. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  5. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  6. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  7. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  8. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  9. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  10. Tebartz C, et al. 2015. J Immunol. 194:1100. PubMed
  11. Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed
  12. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  13. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  14. Shen H, et al. 2015. Infect Immun . 83: 2694 - 2704. PubMed
  15. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  16. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  17. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
  18. Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed
  19. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  20. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  21. Arce-Cerezo A, et al. 2015. Sci Rep. 5: 1448. PubMed
  22. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  23. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  24. Groppa E, et al. 2015. EMBO Mol Med. 2015; 7: 1366 - 1384. PubMed
  25. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  26. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  27. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  28. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  29. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  30. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  31. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  32. Kirschbaum K, et al. 2016. Proc Natl Acad Sci U S A. 113: 13227 - 13232. PubMed
  33. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  34. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  35. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  36. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  37. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  38. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  39. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  40. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  41. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  42. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  43. Gao P, et al. 2020. Front Immunol. 1.636111111. PubMed
  44. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  45. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  46. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  47. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  48. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  49. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  50. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  51. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  52. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  53. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  54. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  55. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  56. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  57. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  58. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  59. Liu K, et al. 2021. Cell Metabolism. 33(3):666-675.e4. PubMed
  60. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  61. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  62. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  63. Young K, et al. 2021. Cell Stem Cell. . PubMed
  64. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  65. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  66. Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed
  67. Gao C, et al. 2017. Mol Neurobiol.. 10.1007/s12035-016-0318-0. PubMed
  68. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  69. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  70. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  71. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  72. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  73. McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed
  74. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  75. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  76. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  77. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  78. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  79. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  80. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  81. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  82. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  83. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  84. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
  85. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  86. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
  87. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  88. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  89. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  90. Saggu G, et al. 2018. Nat Commun. 9:5058. PubMed
  91. Akhmetzyanova ER, et al. 2019. Front Cell Neurosci. 12:507. PubMed
  92. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  93. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  94. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  95. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  96. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  97. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  98. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  99. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  100. Saul S, et al. 2019. J Leukoc Biol. N/A. PubMed
  101. Lalor R, et al. 2019. Nutrients. 11. PubMed
  102. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
  103. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  104. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  105. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  106. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  107. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  108. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  109. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  110. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  111. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  112. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  113. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  114. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  115. Gibson EM, et al. 2019. Cell. 176:43:00. PubMed
  116. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  117. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  118. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  119. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  120. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  121. Secondini C, et al. 2017. Oncoimmunology. 6:e1316437. PubMed
  122. Vasilijevic J, et al. 2017. PLoS Pathog. 13:e1006650. PubMed
  123. Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed
  124. Okunuki Y, et al. 2018. Proc Natl Acad Sci U S A. 115:E6264. PubMed
  125. Rosen DA, et al. 2019. Sci Transl Med. 11:eaau5266. PubMed
  126. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  127. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  128. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  129. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  130. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  131. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  132. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  133. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  134. Dokoshi T, et al. 2020. Cell Rep. 30:61. PubMed
  135. Wilson DH, et al. 2020. Nat Commun. 0.767361111. PubMed
  136. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  137. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  138. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  139. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  140. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  141. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  142. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  143. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  144. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  145. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  146. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  147. Endoh M, et al. 2020. Cell Reports. 30(6):1823-1834. PubMed
  148. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  149. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  150. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  151. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  152. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  153. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  154. Alfaro M, et al. 2008. Proc Natl Acad Sci U S A. 105:18366. PubMed
  155. Meeks K, et al. 2009. J Immunol. 183:8026. PubMed
  156. Beauchamp N, et al. 2010. J Virol. 84:10191. PubMed
RRID
AB_312798 (BioLegend Cat. No. 101215)
AB_312799 (BioLegend Cat. No. 101216)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account