PE anti-human/mouse/rat CD205 (DEC-205) Antibody

Pricing & Availability
Clone
HD83 (See other available formats)
Regulatory Status
RUO
Other Names
LY75
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HD83_PE_CD205_Antibody_FC_1_082013.jpg
Human peripheral blood monocyte-derived dendritic cells were stained with CD209 APC and CD205 (clone HD83) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • HD83_PE_CD205_Antibody_FC_1_082013.jpg
    Human peripheral blood monocyte-derived dendritic cells were stained with CD209 APC and CD205 (clone HD83) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • HD83_PE_CD205_Antibody_FC_2_082013.jpg
See PE spectral data
Cat # Size Price Quantity Check Availability Save
359203 25 tests £86.00
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359204 100 tests £202.00
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Description

CD205, also known as DEC-205 and LY75, belongs to the macrophage mannose receptor (MMR) family of C-type lectins. Both human and mouse CD205 share multiple similar structures: an N-terminal cysteine-rich domain, a fibronectin type II domain, and a series of 10 tandemly repeated C-type like domains (CTLDs). CD205 is highly expressed on dendritic cells and thymic cortical epithelial cells in humans and mice. It also can be detected on T and B cells, monocytes, neutrophils, and epithelial cells. CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing areas. It mediates antigen uptake and presentation and cross-presentation to T cells. It has been reported that CD205 acts as a recognition receptor for dying cells, which potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human , Mouse, Rat, Cross Reactivity: Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hDEC205-hIgG1Fc protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1 and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)
Product Citations
  1. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
RRID
AB_2562438 (BioLegend Cat. No. 359203)
AB_2562439 (BioLegend Cat. No. 359204)

Antigen Details

Structure
An N-terminal cysteine-rich domain, a fibronectin type II domain and a series of 10 tandemnly repeated C-type like domains (CTLDs)
Distribution
Expressed at high levels by dendritic cells and thymic epithelial cells; also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells, and epithelial cells
Function
Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells
Cell Type
Dendritic cells, Epithelial cells, B cells, Macrophages, Langerhans cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Jiang W, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.

Gene ID
4065 View all products for this Gene ID 17076 View all products for this Gene ID 499800 View all products for this Gene ID
UniProt
View information about CD205 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/21/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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