- HCD14 (See other available formats)
- Regulatory Status
- Other Names
- LPS receptor
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
(PubMed link indicates BioLegend citation)
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Product Citations
AB_830688 (BioLegend Cat. No. 325615)
AB_830689 (BioLegend Cat. No. 325616)
- GPI-linked membrane glycoprotein, 53-55 kD
Monocytes, macrophages, granulocytes (low)
- LPS receptor, clearance of Gram-negative pathogens
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- View information about CD14 on UniProt.org
|PerCP anti-human CD14||HCD14||FC|
|Purified anti-human CD14||HCD14||FC, ICC|
|FITC anti-human CD14||HCD14||FC|
|PE anti-human CD14||HCD14||FC|
|APC anti-human CD14||HCD14||FC|
|Alexa Fluor® 488 anti-human CD14||HCD14||FC|
|Alexa Fluor® 647 anti-human CD14||HCD14||FC, ICC|
|Alexa Fluor® 700 anti-human CD14||HCD14||FC|
|Pacific Blue™ anti-human CD14||HCD14||FC|
|PE/Cyanine7 anti-human CD14||HCD14||FC|
|APC/Cyanine7 anti-human CD14||HCD14||FC|
|PerCP/Cyanine5.5 anti-human CD14||HCD14||FC|
|Biotin anti-human CD14||HCD14||FC, Cell Sep - Pos|
|Brilliant Violet 421™ anti-human CD14||HCD14||FC|
|Alexa Fluor® 594 anti-human CD14||HCD14||ICC, FC|
|PE/Dazzle™ 594 anti-human CD14||HCD14||FC|
|Spark Blue™ 574 anti-human CD14||HCD14||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PerCP anti-human CD14
Purified anti-human CD14
FITC anti-human CD14
PE anti-human CD14
APC anti-human CD14
Alexa Fluor® 488 anti-human CD14
Alexa Fluor® 647 anti-human CD14
Alexa Fluor® 700 anti-human CD14
Pacific Blue™ anti-human CD14
PE/Cyanine7 anti-human CD14
APC/Cyanine7 anti-human CD14
PerCP/Cyanine5.5 anti-human CD14
Biotin anti-human CD14
Brilliant Violet 421™ anti-human CD14
Alexa Fluor® 594 anti-human CD14
PE/Dazzle™ 594 anti-human CD14
Spark Blue™ 574 anti-human CD14