Brilliant Violet 421™ anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1A8_BV421_032911
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 1A8_BV421_032911
    C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 1A8_BV421_Ly-6G_Antibody2_IHC_122916
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS and 5% rat/mouse serum for one hour at room temperature. Then the section was stained with 2.5 µg/mL of Ly-6G (clone 1A8) Brilliant Violet 421™ (red), 5 µg/mL of CD3 Alexa Fluor® 647 (green), and 5 µg/mL of B220 Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data See high resolution IHC-F data...
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127627 125 µL £117
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127628 50 µg £174
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  2. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  3. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  4. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  5. Nepal S, et al. 2019. Proc Natl Acad Sci U S A. 116:16513. PubMed
  6. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  7. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  8. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  9. Green DP, et al. 2019. Neuron. 101:412. PubMed
  10. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  11. Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed
  12. Wang L, et al. 2020. Cells. 0.458333333. PubMed
  13. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  14. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  15. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  16. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  17. Köhler D, et al. 2020. Nat Commun. 11:1315. PubMed
  18. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  19. Baudoux T, et al. 2018. Sci Rep. 4.0375. PubMed
  20. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  21. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  22. Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed
  23. Kennedy J, et al. 2014. J Exp Med. 211:2519. PubMed
  24. Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed
  25. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  26. Kullberg M, et al. 2015. Nanomedicine. 11: 1355-1363. PubMed
  27. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  28. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  29. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  30. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  31. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  32. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  33. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  34. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  35. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  36. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  37. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  38. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
RRID
AB_10897944 (BioLegend Cat. No. 127627)
AB_2562567 (BioLegend Cat. No. 127628)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F
Purified anti-mouse Ly-6G 1A8 FC, IHC-F, IHC
Biotin anti-mouse Ly-6G 1A8 FC, IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC, IHC-F
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC, IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC, IHC-F, SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC, IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC, Depletion, IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC, CyTOF®, WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Go To Top Version: 5    Revision Date: 01/06/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 594 anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G

  • Biotin anti-mouse Ly-6G

  • FITC anti-mouse Ly-6G

  • PE anti-mouse Ly-6G

  • Alexa Fluor® 647 anti-mouse Ly-6G

  • Pacific Blue™ anti-mouse Ly-6G

  • APC anti-mouse Ly-6G

  • PerCP/Cyanine5.5 anti-mouse Ly-6G

  • PE/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 700 anti-mouse Ly-6G

  • APC/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 488 anti-mouse Ly-6G

  • Brilliant Violet 421™ anti-mouse Ly-6G

  • Brilliant Violet 570™ anti-mouse Ly-6G

  • Ultra-LEAF™ Purified anti-mouse Ly-6G

  • Brilliant Violet 510™ anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G (Maxpar® Ready)

  • Brilliant Violet 650™ anti-mouse Ly-6G

  • Brilliant Violet 711™ anti-mouse Ly-6G

  • Brilliant Violet 605™ anti-mouse Ly-6G

  • Brilliant Violet 785™ anti-mouse Ly-6G

  • PE/Dazzle™ 594 anti-mouse Ly-6G

  • APC/Fire™ 750 anti-mouse Ly-6G

  • PerCP anti-mouse Ly-6G

  • TotalSeq™-A0015 anti-mouse Ly-6G

  • TotalSeq™-C0015 anti-mouse Ly-6G

  • TotalSeq™-B0015 anti-mouse Ly-6G

  • Spark Blue™ 550 anti-mouse Ly-6G

  • Spark NIR™ 685 anti-mouse Ly-6G

  • Spark YG™ 593 anti-mouse Ly-6G

  • PE/Cyanine5 anti-mouse Ly-6G

  • PE/Fire™ 810 anti-mouse Ly-6G Antibody

  • Spark UV™ 387 anti-mouse Ly-6G

  • PE/Fire™ 640 anti-mouse Ly-6G

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