- 11F6 (See other available formats)
- Regulatory Status
- Other Names
- Mki67, Ki67, Ki-67, MIB-1, KIA
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.Product Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Host Species
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
AB_2716014 (BioLegend Cat. No. 151209)
AB_2716008 (BioLegend Cat. No. 151210)
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Nucleus and chromosomes.
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- View information about Ki-67 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
|Purified anti-mouse/human Ki-67||11F6||IHC-F, ICC|
|Alexa Fluor® 488 anti-mouse/human Ki-67||11F6||ICFC, ICC, IHC-F|
|Alexa Fluor® 647 anti-mouse/human Ki-67||11F6||ICFC, ICC, IHC-F|
|Brilliant Violet 421™ anti-mouse/human Ki-67||11F6||ICFC, IHC-F|
|PE anti-mouse/human Ki-67||11F6||ICFC|
|Alexa Fluor® 594 anti-mouse/human Ki-67||11F6||IHC-F|
|FITC anti-mouse/human Ki-67||11F6||ICFC|
|Brilliant Violet 650™ anti-mouse/human Ki-67||11F6||ICFC|
|PE/Cyanine7 anti-mouse/human Ki-67||11F6||ICFC|
|PE/Dazzle™ 594 anti-mouse/human Ki-67||11F6||ICFC|
|PerCP/Cyanine5.5 anti-mouse/human Ki-67||11F6||ICFC|
|Pacific Blue™ anti-mouse/human Ki-67||11F6||ICFC|
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse/human Ki-67
Alexa Fluor® 488 anti-mouse/human Ki-67
Alexa Fluor® 647 anti-mouse/human Ki-67
Brilliant Violet 421™ anti-mouse/human Ki-67
PE anti-mouse/human Ki-67
Alexa Fluor® 594 anti-mouse/human Ki-67
FITC anti-mouse/human Ki-67
Brilliant Violet 650™ anti-mouse/human Ki-67
PE/Cyanine7 anti-mouse/human Ki-67
PE/Dazzle™ 594 anti-mouse/human Ki-67
PerCP/Cyanine5.5 anti-mouse/human Ki-67
Pacific Blue™ anti-mouse/human Ki-67