FITC Annexin V

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Regulatory Status
RUO
Other Names
Annexin A5
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Product Citations
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AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
See FITC spectral data
Cat # Size Price Quantity Avail. Save
640905 25 tests 56,00€
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640906 100 tests 132,00€
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640945 300 tests 276,00€
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Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical data sheet

Product Details

Reactivity
All mammalian species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
2. Transfer 100 µL of cell suspension in 5 ml test tube.
3. Add 5 µL of FITC Annexin V.
4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404).
5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. Analyze by flow cytometry.


For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References

(PubMed link indicates BioLegend citation)
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RRID
not an antibody (BioLegend Cat. No. 640905)
not an antibody (BioLegend Cat. No. 640906)
not an antibody (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

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Recombinant Human IL-4 (Animal-Free) BA
LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit ELISA
LEGEND MAX™ Human Soluble CD137/4-1BB ELISA Kit with Pre-coated Plates ELISA
LEGEND MAX™ Total TGF-β1 ELISA Kit ELISA
LEGEND MAX™ Free Active TGF-β1 ELISA Kit ELISA
LEGEND MAX™ Human anti-mouse Ig (HAMA) ELISA Kit ELISA
LEGEND MAX™ Rat TNF-α ELISA Kit ELISA
Alexa Fluor® 647 Annexin V FC
Recombinant Human IL-22 (carrier-free) BA
ELISA MAX™ Deluxe Set Human IL-21 ELISA
Recombinant Mouse IL-4 (carrier-free) BA
ELISA MAX™ Deluxe Set Mouse IL-12 (p70) ELISA
ELISA MAX™ Deluxe Set Mouse IL-23 ELISA
Recombinant Human IL-10 (mammalian expressed, carrier-free) BA
ELISA MAX™ Deluxe Set Mouse TSLP ELISA
ELISA MAX™ Deluxe Set Human IL-1β ELISA
Recombinant Mouse IL-6 (ELISA Std.) ELISA
Recombinant Mouse IL-33 (carrier-free) BA
LEGEND MAX™ Human MCP-1/CCL2 ELISA Kit ELISA
Recombinant Human IL-33 (ELISA Std.) ELISA
Recombinant Rat IL-2 (carrier-free) BA
Recombinant Human IL-1β (carrier-free) BA
Recombinant Human IL-4 (ELISA Std.) ELISA
Recombinant Human IL-1α (carrier-free) BA
Recombinant Mouse IL-1α (carrier-free) BA
Recombinant Mouse IL-1β (carrier-free) BA
Recombinant Human IL-3 (Animal-Free) BA
Recombinant Human IL-1RA (Animal-Free) BA
LEGEND MAX™ Mouse TNF-α ELISA Kit ELISA
Recombinant Mouse IL-13 (carrier-free) BA
Recombinant Mouse FGF-basic (carrier-free) BA
ELISA MAX™ Deluxe Set Human IL-8 ELISA
ELISA MAX™ Deluxe Set Human IL-9 ELISA
ELISA MAX™ Standard Set Human IL-8 ELISA
Recombinant Mouse IL-1α (ELISA Std.) ELISA
ELISA MAX™ Deluxe Set Human IL-15 ELISA
Recombinant Human IL-8 (ELISA Std.) ELISA
Recombinant Human IL-13 (carrier-free) BA
ELISA MAX™ Deluxe Set Human TSLP ELISA
Recombinant Human IL-10 (carrier-free) BA
Recombinant Mouse IL-2 (ELISA Std.) ELISA
Recombinant Human IL-9 (ELISA Std.) ELISA
ELISA MAX™ Deluxe Set Human IL-22 ELISA
Recombinant Mouse IL-10 (carrier-free) BA
LEGEND MAX™ Mouse IL-21 ELISA Kit with Pre-coated Plates ELISA
LEGEND MAX™ Human IL-9 ELISA Kit ELISA
LEGEND MAX™ Human IL-27 ELISA Kit ELISA
LEGEND MAX™ Human TSLP ELISA Kit ELISA
LEGEND MAX™ Mouse IL-23 (p19/p40) ELISA Kit ELISA
LEGEND MAX™ Mouse IL-17A ELISA Kit ELISA
LEGEND MAX™ Mouse GM-CSF ELISA Kit ELISA
LEGEND MAX™ Human GM-CSF ELISA Kit ELISA
LEGEND MAX™ Human IL-2 ELISA Kit ELISA
LEGEND MAX™ Human IL-8 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-6 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-2 ELISA Kit ELISA
LEGEND MAX™ Mouse IFN-γ ELISA Kit ELISA
LEGEND MAX™ Human IL-10 ELISA Kit ELISA
LEGEND MAX™ Human IL-6 ELISA Kit ELISA
LEGEND MAX™ Human TNF-α ELISA Kit ELISA
LEGEND MAX™ Human IFN-γ ELISA Kit ELISA
LEGEND MAX™ Human IL-28A ELISA Kit with Pre-coated Plates ELISA
LEGEND MAX™ Human IL-4 ELISA Kit ELISA
LEGEND MAX™ Human IL-15 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-12 (p70) ELISA Kit ELISA
LEGEND MAX™ Mouse IL-4 ELISA Kit ELISA
LEGEND MAX™ Human IL-22 ELISA Kit ELISA
Recombinant Human IL-17A/F Heterodimer (carrier-free) BA
Recombinant Rat TNF-α (carrier-free) BA
Recombinant Human CCL5 (RANTES) (Animal-Free) BA
Recombinant Human CCL5 (RANTES) (carrier-free) BA
Recombinant Human LT-α (TNF-β) ELISA, BA
Recombinant Mouse IFN-β1 (carrier-free) BA
Recombinant Mouse VEGF-120 (carrier-free) BA
Recombinant Mouse IL-10 (ELISA Std.) ELISA
Recombinant Human PDGF-BB (carrier-free) BA
Recombinant Human CCL2 (MCP-1) (carrier-free) BA
Recombinant Mouse M-CSF (carrier-free) BA
Recombinant Human CXCL12 (SDF-1α) (carrier-free) BA
Recombinant Mouse IL-3 (carrier-free) BA
Recombinant Human GM-CSF (carrier-free) BA
Recombinant Mouse SCF (carrier-free) BA
LEGEND MAX™ Mouse IL-22 ELISA Kit ELISA
LEGEND MAX™ Human IL-33 ELISA Kit ELISA
LEGEND MAX™ Human IL-17F ELISA Kit ELISA
LEGEND MAX™ Mouse IL-34 ELISA Kit ELISA
FOXP3 Fix/Perm Buffer (4x) ICFC
Recombinant Human IL-1α (ELISA Std.) ELISA
Recombinant Human IL-17F (carrier-free) BA
Recombinant Human IL-10 (ELISA Std.) ELISA
Recombinant Human IL-13 (ELISA Std.) ELISA
Recombinant Human IL-21 (ELISA Std.) ELISA
Recombinant Human IL-5 (ELISA Std.) ELISA
Recombinant Human IL-12/IL-23 (p40) (ELISA Std.) ELISA
Recombinant Human GM-CSF (ELISA Std.) ELISA
Recombinant Mouse IL-1β (ELISA Std.) ELISA
Recombinant Mouse IL-4 (ELISA Std.) ELISA
Recombinant Mouse IL-17A (ELISA Std.) ELISA
Recombinant Mouse MCP-1 (ELISA Std.) ELISA
Recombinant Mouse IL-12/IL-23 (p40) (ELISA Std.) ELISA
Recombinant Mouse IL-12 (p70) (ELISA Std.) ELISA
Recombinant Human IL-1β (ELISA Std.) ELISA
Human TruStain FcX™ (Fc Receptor Blocking Solution) FC, ICC, ICFC
Recombinant Mouse IL-12 (p70) (carrier-free) BA
Recombinant Mouse IL-17F (carrier-free) BA
Recombinant Human IL-12 (p70) (carrier-free) BA
Recombinant Human EGF (Animal-Free) BA
Recombinant Human IFN-γ (Animal-Free) BA
LEGEND MAX™ Human IL-12 (p70) ELISA Kit ELISA
LEGEND MAX™ Human MRP8/14 (Calprotectin) ELISA Kit ELISA
Recombinant Human TWEAK (CD255) (carrier-free) BA
Recombinant Mouse IL-15 (carrier-free) BA
Recombinant Human IL-4 (carrier-free) BA
LEGEND MAX™ Human FGL2 ELISA Kit ELISA
LEGEND MAX™ Human IL-13 ELISA Kit ELISA
LEGEND MAX™ Human IL-12/IL-23 (p40) ELISA Kit ELISA
LEGEND MAX™ Mouse IL-17F ELISA Kit ELISA
LEGEND MAX™ Mouse IL-33 ELISA Kit ELISA
Recombinant Human IL-23 (carrier-free) BA
Recombinant Human IL-8 (carrier-free) BA
Recombinant Human IL-2 (carrier-free) BA
Recombinant Mouse IL-27 (carrier-free) BA
Recombinant Mouse IL-12 p40 Homodimer (carrier-free) BA
ELISA MAX™ Deluxe Set Mouse IL-22 ELISA
DyLight™ 488 Streptavidin FC, ICC, IHC-F, IHC-P
DyLight™ 649 Streptavidin FC, ICC, IHC-F, IHC-P
LEGEND MAX™ Rat IL-6 ELISA Kit ELISA
Recombinant Mouse EGF (Animal-Free) BA
Recombinant Mouse FGF-basic (Animal-Free) BA
Recombinant Mouse G-CSF (Animal-Free) BA
Recombinant Human VEGF-165 (Animal-Free) BA
Recombinant Human TNF-α (Animal-Free) BA
Recombinant Human TRANCE (RANKL) (Animal-Free) BA
Recombinant Human IL-27 (carrier-free) BA
LEGEND MAX™ Human IL-1β ELISA Kit ELISA
Recombinant Mouse IL-34 (carrier-free) BA
Recombinant Human TGF-α (Animal-Free) BA
Recombinant Human Thrombopoietin (TPO) (Animal-Free) BA
Recombinant Human GM-CSF (Animal-Free) BA
LEGEND MAX™ Mouse IL-17A/F ELISA Kit ELISA
LEGEND MAX™ Rat IL-17A ELISA Kit ELISA
Recombinant Human Oncostatin M (Animal-Free) BA
Recombinant Human PDGF-AA (Animal-Free) BA
ELISA MAX™ Deluxe Set Rat TNF-a ELISA
ELISA MAX™ Deluxe Set Rat IL-17A ELISA
Brilliant Violet 421™ Streptavidin FC, ICFC, ICC, IHC-F, IHC-P
Recombinant Mouse TACI-Fc Chimera (carrier-free) BA
FOXP3 Perm Buffer (10x) ICFC
Recombinant Mouse IL-7 (carrier-free) BA
Recombinant Human IL-34 (carrier free) BA
Recombinant Human M-CSF (Animal-Free) BA
Recombinant Human KGF (FGF-7) (Animal-Free) BA
Recombinant Human LIF (Animal-Free) BA
RBC Lysis/Fixation Solution (10X) FC
Recombinant Mouse CXCL9 (MIG) (carrier-free) BA
LEGEND MAX™ Rat IFN-γ ELISA Kit ELISA
LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit ELISA
LEGEND MAX™ Human Active MIF ELISA Kit ELISA
LEGEND MAX™ Human IL-17A/F ELISA Kit ELISA
Brilliant Violet 570™ Streptavidin FC, ICFC
Recombinant Human CXCL9 (MIG) (carrier-free) BA
Recombinant Human IL-3 (carrier-free) BA
ELISA MAX™ Deluxe Set Human Mesothelin ELISA
Recombinant Human IL-36γ (Animal-Free) BA
Recombinant Human CXCL1 (GRO-α) (carrier-free) BA
ELISA MAX™ Deluxe Set Human IL-17A ELISA
LEGEND MAX™ Human IL-17A ELISA Kit ELISA
Recombinant Mouse IL-21 (carrier-free) BA
Recombinant Mouse CXCL5 (LIX) (carrier-free) BA
Recombinant Human CXCL5 (ENA-78) (carrier-free) BA
Recombinant Human CXCL10 (IP-10) (carrier-free) BA
Recombinant Human IL-12 (p70) (ELISA Std.) ELISA
Recombinant Human IL-22 (Animal-Free) BA
Recombinant Human IL-33 (Animal-Free) BA
Recombinant Mouse CXCL10 (IP-10) (carrier-free) BA
Recombinant Mouse CXCL1 (KC) (carrier-free) BA
Recombinant Human SCF (carrier-free) BA
Recombinant Mouse G-CSF (carrier-free) BA
Recombinant Human CXCL13 (carrier-free) BA
ELISA MAX™ Deluxe Set Human MCP-1/CCL2 ELISA
Recombinant Human M-CSF (carrier-free) BA
Recombinant Human CXCL11 (ITAC) (carrier-free) BA
Recombinant Mouse CXCL11 (ITAC) (carrier-free) BA
Recombinant Mouse CCL2 (MCP-1) (carrier-free) BA
Recombinant Human G-CSF (carrier-free) BA
Recombinant Mouse CXCL12 (SDF-1α) (carrier-free) BA
Recombinant Mouse CCL17 (TARC) (carrier-free) BA
Recombinant Mouse IL-5 (carrier-free) BA
LEGEND MAX™ Mouse Latent TGF-β ELISA Kit ELISA
LEGEND MAX™ Human Granzyme B ELISA Kit ELISA
Recombinant Human CCL8 (MCP-2) (carrier-free) BA
Recombinant Mouse CCL8 (MCP-2) (carrier-free) BA
Brilliant Violet 605™ Streptavidin FC, ICFC
Brilliant Violet 650™ Streptavidin FC, ICFC
Recombinant Mouse ICAM-1-Fc Chimera (carrier-free) BA
Recombinant Human IL-33 (carrier-free) BA
Recombinant Human IL-7 (carrier-free) BA
Recombinant Human CXCL2 (Groβ) (carrier-free) BA
Recombinant Human CCL19 (MIP-3β) (carrier-free) BA
Recombinant Human CCL21 (6CKine) (carrier-free) BA
Recombinant Mouse CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Mouse IFN-β ELISA Kit ELISA
LEGEND MAX™ Human APRIL/TNFSF13 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-10 ELISA Kit ELISA
DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) ICFC, ICC
Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Mid Range Intensity FC
Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm) FC
Rainbow Calibration Particles, 6 peaks (3.0-3.4 µm) FC
Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Bright Intensity FC
Cyto-Last™ Buffer ICFC
Recombinant Human TSLP (carrier-free) BA
Recombinant Mouse CXCL2 (MIP-2) (carrier-free) BA
Recombinant Human CCL1 (I-309) (carrier-free) BA
ELISA MAX™ Deluxe Set Mouse IL-10 ELISA
ELISA MAX™ Standard Set Mouse IL-10 ELISA
Recombinant Mouse CCL11 (Eotaxin) (carrier-free) BA
Recombinant Human CCL11 (Eotaxin) (carrier-free) BA
Recombinant Mouse VEGF-164 (carrier-free) BA
Recombinant Human VEGF-121 (carrier-free) BA
Recombinant Human TGF-β2 (carrier-free) BA
LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit ELISA
LEGEND MAX™ Human IL-34 ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human IL-34 ELISA
Brilliant Violet 510™ Streptavidin FC, ICFC
APC Annexin V FC
PE Annexin V FC
Recombinant Human CX3CL1 (Fractalkine) (carrier-free) BA
Recombinant Mouse CX3CL1 (Fractalkine) (carrier-free) BA
Recombinant Human CCL27 (CTACK) (carrier-free) BA
Recombinant Human CCL22 (MDC) (carrier-free) BA
FITC Annexin V Apoptosis Detection Kit with PI FC
Recombinant Mouse CCL24 (Eotaxin-2) (carrier-free) BA
Recombinant Human CCL24 (Eotaxin-2) (carrier-free) BA
Recombinant Human VEGF-165 (carrier-free) BA
Recombinant Human CCL28 (MEC) (carrier-free) BA
Recombinant Mouse CCL28 (MEC) (carrier-free) BA
Recombinant Mouse CCL1 (I-309) (carrier-free) BA
LEGEND MAX™ Human Myeloperoxidase ELISA Kit ELISA
Recombinant Human CCL20 (MIP-3α) (carrier-free) BA
FITC Annexin V Apoptosis Detection Kit with 7-AAD FC
Recombinant Mouse IL-33 (ELISA Std.) ELISA
Recombinant Human IL-22 (ELISA Std.) ELISA
Recombinant Human CXCL17 (VCC-1) (carrier-free) BA
Recombinant Mouse CXCL17 (VCC-1) (carrier-free) BA
Recombinant Human EGF (carrier-free) BA
Recombinant Mouse EGF (carrier-free) BA
Recombinant Mouse IL-6 (Animal-Free) BA
Recombinant Human TGF-β3 (carrier-free) BA
Recombinant Human IL-11 (carrier-free) BA
Recombinant Mouse CCL7 (MCP-3) (carrier-free) BA
Recombinant Mouse CCL9 (MIP-1γ) (carrier-free) BA
Recombinant Mouse CCL21 (6CKine) (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (Animal-Free) BA
Recombinant Mouse IL-3 (Animal-Free) BA
Recombinant Mouse IL-4 (Animal-Free) BA
Recombinant Human IL-21 (carrier-free) BA
Recombinant Mouse CXCL13 (carrier-free) BA
LEGEND MAX™ Human sICAM-1/CD54 ELISA Kit with Pre-coated Plates ELISA
Stop Solution for TMB Substrate ELISA
Zombie Aqua™ Fixable Viability Kit FC, ICFC, ICC
LEGEND MAX™ Human CXCL10 (IP-10) ELISA Kit ELISA
Recombinant Mouse CXCL7 (NAP-2) (carrier-free) BA
Recombinant Mouse CXCL3 (carrier-free) BA
Recombinant Mouse CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL23 (MPIF-1) (Arg46-Asn120) (carrier-free) BA
Recombinant Human Erythropoietin (EPO) (carrier-free) BA
Recombinant Mouse IL-25 (IL-17E) (insect expressed, carrier-free) BA
Recombinant Human FASL (TNFSF6) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) ELISA
Recombinant Mouse CXCL14 (BRAK) (carrier-free) BA
Recombinant Human CXCL14 (BRAK) (carrier-free) BA
Recombinant Mouse Erythropoietin (EPO) (carrier-free) BA
Recombinant Human CXCL12 (SDF-1β) (carrier free) BA
Recombinant Mouse CCL19 (MIP-3β) (carrier-free) BA
Recombinant Mouse CCL12 (MCP-5) (carrier-free) BA
Zombie NIR™ Fixable Viability Kit FC, ICFC
Recombinant Human VEGF-C (carrier-free) BA
Recombinant Mouse NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Human NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Mouse IGF-II (carrier-free) BA
Purified Annexin V FC
Recombinant Mouse CXCL12 (SDF-1β) (carrier-free) BA
Recombinant Human TGF-α (carrier-free) BA
Recombinant Rat CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Human CCL17 (TARC) ELISA Kit ELISA
LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit ELISA
Recombinant Mouse CXCL4 (PF-4) (carrier-free) BA
Accutase® Cell Detachment Solution FC
Recombinant Human IGF-II (carrier-free) BA
Recombinant Human IGF-I (carrier-free) BA
Recombinant Human TRANCE (RANKL) (carrier-free) BA
Recombinant Mouse BAFF (carrier-free) BA
Recombinant Human APRIL (TNFSF13) (ELISA Std.) ELISA
Recombinant Human IL-15 (ELISA Std.) ELISA
Recombinant Mouse IGF-I (carrier-free) BA
Recombinant Human sTNF-RII (TNFRSF1B) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL17 (TARC) ELISA
Recombinant Mouse CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Human CD40L (TNFSF5) (carrier-free) BA
Recombinant Human CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Mouse TNF-α (Animal-Free) BA
Recombinant Human Activin A (carrier-free) BA
Recombinant Human FGF-4 (carrier-free) BA
Recombinant Human IL-18 (carrier-free) BA
Recombinant Rat GM-CSF (carrier-free) BA
Recombinant Human IFN-α2 (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL5 (RANTES) ELISA
ELISA MAX™ Deluxe Set Human CXCL5 ELISA
LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human CCL28 ELISA
Recombinant Rat Erythropoietin (EPO) (carrier-free) BA
Recombinant Human PCSK9 (carrier-free) BA
Recombinant Human CCL17 (TARC) (ELISA Std.) ELISA
Recombinant Human TIMP-1 (carrier-free) BA
Recombinant Human Arginase I (carrier-free) BA
Recombinant Human MMP-1 (carrier-free) BA
Recombinant Human CD14 (carrier-free) BA
Recombinant Human CTLA-4 (ELISA Std.) ELISA
Recombinant Mouse IL-34 (ELISA Std.) ELISA
Recombinant Human IL-34 (ELISA Std.) ELISA
Recombinant Human Latent TGF-β (ELISA Std.) ELISA
Recombinant Human Mesothelin-Fc Chimera (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (carrier-free) BA
Recombinant Human TIMP-2 (carrier-free) BA
Recombinant Mouse TIMP-1 (carrier-free) BA
Brilliant Violet 421™ Annexin V FC
Alexa Fluor® 488 Streptavidin FC, ICFC
Alexa Fluor® 647 Streptavidin FC, ICFC
Recombinant Mouse CCL3 (MIP-1α) (carrier-free) BA
Recombinant Human LIF (carrier-free) BA
Recombinant Mouse Mesothelin (carrier-free) BA
Recombinant Rat IFN-γ (ELISA Std.) ELISA
Recombinant Mouse IL-17A/F Heterodimer (ELISA Std.) ELISA
Recombinant Human TGF-β1 (ELISA Std.) ELISA
Zombie Violet™ Fixable Viability Kit FC, ICFC, ICC
Zombie Green™ Fixable Viability Kit FC, ICFC, ICC
Zombie Red™ Fixable Viability Kit FC, ICFC, ICC
Zombie UV™ Fixable Viability Kit FC, ICFC
CFSE Cell Division Tracker Kit ICFC, in-vivo Cell Tracking
LEGEND MAX™ Human Adiponectin (Acrp30) ELISA Kit ELISA
Bromodeoxyuridine (BrdU) FC, ICFC
Recombinant Mouse CCL5 (RANTES) (carrier-free) BA
Recombinant Mouse IFN-β (ELISA Std.) ELISA
Recombinant Human IL-9 (carrier-free) BA
Recombinant Mouse Latent TGF-β (ELISA Std.) ELISA
Recombinant Human MMP-3 (carrier-free) BA
APC Streptavidin (High Concentration) FC, ICFC
Recombinant Human CCL2 (MCP-1) (Animal-Free) BA
Recombinant Human VEGFR2-Fc Chimera (carrier-free) BA
Recombinant Human NT-4 (Animal-Free) BA
Cell Activation Cocktail (without Brefeldin A) Activ, FC, ICFC
Recombinant Human RBP4 (carrier-free) BA
Recombinant Rat IFN-γ (carrier-free) BA
Recombinant Human ICOS-Fc Chimera (carrier-free) BA
Cell Activation Cocktail (with Brefeldin A) Activ, FC, ICFC
Recombinant Mouse ICOS-Fc Chimera (carrier-free) BA
Recombinant Mouse VEGF-164 (Animal-Free) BA
Recombinant Human NT-3 (carrier-free) BA
Recombinant Human NT-4 (carrier-free) BA
Recombinant Human FGF-1-acidic (carrier-free) BA
Nunc™ MaxiSorp™ ELISA Plates, Uncoated ELISA
Plate Sealers ELISA
DRAQ7™ FC, ICFC
Recombinant Human WISP-1 (carrier-free) BA
Recombinant Human WNT-7a (carrier-free) BA
Recombinant Human BMP-4 (carrier-free) BA
Go To Top Version: 5    Revision Date: 04.20.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • LEGEND MAX™ Human Mesothelin ELISA Kit

  • Zombie Yellow™ Fixable Viability Kit

  • LEGENDplex™ Mouse CCL3 (MIP-1α) Capture Bead B4, 13X

  • ELISA MAX™ Deluxe Set Mouse IL-17A/F

  • FITC Avidin

  • TMB Substrate Set

  • Recombinant Human IL-5

  • APC Streptavidin

  • APC/Cyanine7 Streptavidin

  • Cyanine5 Streptavidin

  • FITC Streptavidin

  • HRP Streptavidin

  • PE Streptavidin

  • PE/Cyanine5 Streptavidin

  • PE/Cyanine7 Streptavidin

  • HRP Avidin

  • Cell Staining Buffer

  • ELISA Assay Diluent (5X)

  • Fixation Buffer

  • Intracellular Staining Permeabilization Wash Buffer (10X)

  • RBC Lysis Buffer (10X)

  • Brefeldin A Solution (1,000X)

  • Monensin Solution (1,000X)

  • 7-AAD Viability Staining Solution

  • Recombinant Human CCL17 (TARC) (carrier-free)

  • Recombinant Mouse IL-36β (carrier-free)

  • Recombinant Human PLGF (carrier-free)

  • ELISA MAX™ Standard Set Human IFN-γ

  • ELISA MAX™ Deluxe Set Human IFN-γ

  • ELISA MAX™ Standard Set Human TNF-α

  • ELISA MAX™ Deluxe Set Human TNF-α

  • ELISA MAX™ Standard Set Human IL-4

  • ELISA MAX™ Deluxe Set Human IL-4

  • ELISA MAX™ Standard Set Human IL-5

  • ELISA MAX™ Deluxe Set Human IL-5

  • ELISA MAX™ Standard Set Human IL-6

  • ELISA MAX™ Deluxe Set Human IL-6

  • ELISA MAX™ Standard Set Human IL-10

  • ELISA MAX™ Deluxe Set Human IL-10

  • ELISA MAX™ Standard Set Human IL-12/IL-23 (p40)

  • ELISA MAX™ Deluxe Set Human IL-12/IL-23 (p40)

  • ELISA MAX™ Standard Set Mouse IFN-γ

  • ELISA MAX™ Deluxe Set Mouse IFN-γ

  • ELISA MAX™ Standard Set Mouse TNF-α

  • ELISA MAX™ Deluxe Set Mouse TNF-α

  • ELISA MAX™ Standard Set Mouse IL-2

  • ELISA MAX™ Deluxe Set Mouse IL-2

  • ELISA MAX™ Standard Set Mouse IL-4

  • ELISA MAX™ Deluxe Set Mouse IL-4

  • ELISA MAX™ Standard Set Mouse IL-5

  • ELISA MAX™ Deluxe Set Mouse IL-5

  • ELISA MAX™ Standard Set Mouse IL-6

  • ELISA MAX™ Deluxe Set Mouse IL-6

  • ELISA MAX™ Standard Set Mouse IL-12/IL-23 (p40)

  • ELISA MAX™ Deluxe Set Mouse IL-12/IL-23 (p40)

  • LEGEND MAX™ Human Latent TGF-β ELISA Kit

  • Propidium Iodide Solution

  • Recombinant Mouse IFN-γ (carrier-free)

  • ELISA MAX™ Standard Set Human IL-12 (p70)

  • ELISA MAX™ Deluxe Set Human IL-12 (p70)

  • ELISA MAX™ Standard Set Human IL-2

  • ELISA MAX™ Deluxe Set Human IL-2

  • Recombinant Human IGF-I (Animal-Free)

  • FOXP3 Fix/Perm Buffer Set

  • ELISA MAX™ Standard Set Human GM-CSF

  • ELISA MAX™ Deluxe Set Human GM-CSF

  • Recombinant Mouse IL-2 (Animal-Free)

  • ELISA MAX™ Standard Set Mouse GM-CSF

  • ELISA MAX™ Deluxe Set Mouse GM-CSF

  • ELISA MAX™ Standard Set Mouse IL-1β

  • ELISA MAX™ Deluxe Set Mouse IL-1β

  • Recombinant Human IL-17A (Animal-Free)

  • Recombinant Human IL-17E (Animal-Free)

  • Recombinant Human IL-15 (Animal-Free)

  • Recombinant Human IL-16 (Animal-Free)

  • Recombinant Mouse IFN-γ (Animal-Free)

  • Recombinant Human G-CSF (Animal-Free)

  • Recombinant Mouse GM-CSF (Animal-Free)

  • Recombinant Human IL-11 (Animal-Free)

  • Pacific Blue™ Annexin V Apoptosis Detection Kit with PI

  • LEGEND MAX™ Mouse TSLP ELISA Kit

  • Recombinant Human IL-10 (Animal-Free)

  • ELISA MAX™ Standard Set Mouse IgE

  • ELISA MAX™ Deluxe Set Mouse IgE

  • Recombinant Human IGF-II (Animal-Free)

  • ELISA MAX™ Standard Set Mouse IL-17A

  • ELISA MAX™ Deluxe Set Mouse IL-17A

  • Recombinant Human IL-9 (Animal-Free)

  • TMB High Sensitivity Substrate Solution

  • Recombinant Human IL-8 (Animal-Free)

  • ELISA MAX™ Standard Set Mouse MCP-1

  • ELISA MAX™ Deluxe Set Mouse MCP-1

  • ELISA Wash Buffer (20X)

  • ELISA Coating Buffer (5X)

  • Recombinant Mouse TRANCE (RANKL) (carrier-free)

  • Pacific Blue™ Annexin V

  • Recombinant Mouse Granzyme B (carrier-free)

  • Recombinant Mouse sTNF-RI (TNFRSF1A) (carrier-free)

  • Recombinant Human TNF-α (carrier-free)

  • Recombinant Human IFN-γ (carrier-free)

  • Recombinant Human IL-15 (carrier-free)

  • PerCP Streptavidin

  • PerCP/Cyanine5.5 Streptavidin

  • Recombinant Human FGF-basic/145aa (carrier-free)

  • Recombinant Mouse IL-6 (carrier-free)

  • Cyanine3 Streptavidin

  • Recombinant Mouse GM-CSF (carrier-free)

  • Recombinant Mouse TNF-α (carrier-free)

  • ELISA MAX™ Deluxe Set Mouse IL-1α

  • ELISA MAX™ Standard Set Mouse IL-1α

  • Recombinant Human IL-2 (ELISA Std.)

  • Recombinant Human TNF-α (ELISA Std.)

  • Recombinant Mouse IL-2 (carrier-free)

  • Recombinant Human IL-17A (carrier-free)

  • Recombinant Human IL-17A (ELISA Std.)

  • Recombinant Mouse IL-23 (carrier-free)

  • Recombinant Human CD40L (TNFSF5) (Animal-Free)

  • LEGEND MAX™ Human IL-21 ELISA Kit

  • Recombinant Human IL-7 (Animal-Free)

  • Recombinant Mouse IFN-γ (ELISA Std.)

  • Recombinant Human IFN-γ (ELISA Std.)

  • Recombinant Human IL-6 (carrier-free)

  • Recombinant Human IL-6 (ELISA Std.)

  • Recombinant Mouse GM-CSF (ELISA Std.)

  • Recombinant Human FGF-basic (146 aa) (Animal-Free)

  • FluoroFix™ Buffer

  • Recombinant Mouse TNF-α (ELISA Std.)

  • Recombinant Human IL-6 (Animal-Free)

  • ELISA MAX™ Deluxe Set Human IL-32α

  • Recombinant Mouse IL-17A (carrier-free)

  • Annexin V Binding Buffer

  • Recombinant Mouse IL-22 (carrier-free)

  • Recombinant Mouse IL-22 (ELISA Std.)

  • Recombinant Human IL-4 (Animal-Free)

  • LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit

  • LEGEND MAX™ Human Soluble CD137/4-1BB ELISA Kit with Pre-coated Plates

  • LEGEND MAX™ Total TGF-β1 ELISA Kit

  • LEGEND MAX™ Free Active TGF-β1 ELISA Kit

  • LEGEND MAX™ Human anti-mouse Ig (HAMA) ELISA Kit

  • LEGEND MAX™ Rat TNF-α ELISA Kit

  • Alexa Fluor® 647 Annexin V

  • Recombinant Human IL-22 (carrier-free)

  • ELISA MAX™ Deluxe Set Human IL-21

  • Recombinant Mouse IL-4 (carrier-free)

  • ELISA MAX™ Deluxe Set Mouse IL-12 (p70)

  • ELISA MAX™ Deluxe Set Mouse IL-23

  • Recombinant Human IL-10 (mammalian expressed, carrier-free)

  • ELISA MAX™ Deluxe Set Mouse TSLP

  • ELISA MAX™ Deluxe Set Human IL-1β

  • Recombinant Mouse IL-6 (ELISA Std.)

  • Recombinant Mouse IL-33 (carrier-free)

  • LEGEND MAX™ Human MCP-1/CCL2 ELISA Kit

  • Recombinant Human IL-33 (ELISA Std.)

  • Recombinant Rat IL-2 (carrier-free)

  • Recombinant Human IL-1β (carrier-free)

  • Recombinant Human IL-4 (ELISA Std.)

  • Recombinant Human IL-1α (carrier-free)

  • Recombinant Mouse IL-1α (carrier-free)

  • Recombinant Mouse IL-1β (carrier-free)

  • Recombinant Human IL-3 (Animal-Free)

  • Recombinant Human IL-1RA (Animal-Free)

  • LEGEND MAX™ Mouse TNF-α ELISA Kit

  • Recombinant Mouse IL-13 (carrier-free)

  • Recombinant Mouse FGF-basic (carrier-free)

  • ELISA MAX™ Deluxe Set Human IL-8

  • ELISA MAX™ Deluxe Set Human IL-9

  • ELISA MAX™ Standard Set Human IL-8

  • Recombinant Mouse IL-1α (ELISA Std.)

  • ELISA MAX™ Deluxe Set Human IL-15

  • Recombinant Human IL-8 (ELISA Std.)

  • Recombinant Human IL-13 (carrier-free)

  • ELISA MAX™ Deluxe Set Human TSLP

  • Recombinant Human IL-10 (carrier-free)

  • Recombinant Mouse IL-2 (ELISA Std.)

  • Recombinant Human IL-9 (ELISA Std.)

  • ELISA MAX™ Deluxe Set Human IL-22

  • Recombinant Mouse IL-10 (carrier-free)

  • LEGEND MAX™ Mouse IL-21 ELISA Kit with Pre-coated Plates

  • LEGEND MAX™ Human IL-9 ELISA Kit

  • LEGEND MAX™ Human IL-27 ELISA Kit

  • LEGEND MAX™ Human TSLP ELISA Kit

  • LEGEND MAX™ Mouse IL-23 (p19/p40) ELISA Kit

  • LEGEND MAX™ Mouse IL-17A ELISA Kit

  • LEGEND MAX™ Mouse GM-CSF ELISA Kit

  • LEGEND MAX™ Human GM-CSF ELISA Kit

  • LEGEND MAX™ Human IL-2 ELISA Kit

  • LEGEND MAX™ Human IL-8 ELISA Kit

  • LEGEND MAX™ Mouse IL-6 ELISA Kit

  • LEGEND MAX™ Mouse IL-2 ELISA Kit

  • LEGEND MAX™ Mouse IFN-γ ELISA Kit

  • LEGEND MAX™ Human IL-10 ELISA Kit

  • LEGEND MAX™ Human IL-6 ELISA Kit

  • LEGEND MAX™ Human TNF-α ELISA Kit

  • LEGEND MAX™ Human IFN-γ ELISA Kit

  • LEGEND MAX™ Human IL-28A ELISA Kit with Pre-coated Plates

  • LEGEND MAX™ Human IL-4 ELISA Kit

  • LEGEND MAX™ Human IL-15 ELISA Kit

  • LEGEND MAX™ Mouse IL-12 (p70) ELISA Kit

  • LEGEND MAX™ Mouse IL-4 ELISA Kit

  • LEGEND MAX™ Human IL-22 ELISA Kit

  • Recombinant Human IL-17A/F Heterodimer (carrier-free)

  • Recombinant Rat TNF-α (carrier-free)

  • Recombinant Human CCL5 (RANTES) (Animal-Free)

  • Recombinant Human CCL5 (RANTES) (carrier-free)

  • Recombinant Human LT-α (TNF-β)

  • Recombinant Mouse IFN-β1 (carrier-free)

  • Recombinant Mouse VEGF-120 (carrier-free)

  • Recombinant Mouse IL-10 (ELISA Std.)

  • Recombinant Human PDGF-BB (carrier-free)

  • Recombinant Human CCL2 (MCP-1) (carrier-free)

  • Recombinant Mouse M-CSF (carrier-free)

  • Recombinant Human CXCL12 (SDF-1α) (carrier-free)

  • Recombinant Mouse IL-3 (carrier-free)

  • Recombinant Human GM-CSF (carrier-free)

  • Recombinant Mouse SCF (carrier-free)

  • LEGEND MAX™ Mouse IL-22 ELISA Kit

  • LEGEND MAX™ Human IL-33 ELISA Kit

  • LEGEND MAX™ Human IL-17F ELISA Kit

  • LEGEND MAX™ Mouse IL-34 ELISA Kit

  • FOXP3 Fix/Perm Buffer (4x)

  • Recombinant Human IL-1α (ELISA Std.)

  • Recombinant Human IL-17F (carrier-free)

  • Recombinant Human IL-10 (ELISA Std.)

  • Recombinant Human IL-13 (ELISA Std.)

  • Recombinant Human IL-21 (ELISA Std.)

  • Recombinant Human IL-5 (ELISA Std.)

  • Recombinant Human IL-12/IL-23 (p40) (ELISA Std.)

  • Recombinant Human GM-CSF (ELISA Std.)

  • Recombinant Mouse IL-1β (ELISA Std.)

  • Recombinant Mouse IL-4 (ELISA Std.)

  • Recombinant Mouse IL-17A (ELISA Std.)

  • Recombinant Mouse MCP-1 (ELISA Std.)

  • Recombinant Mouse IL-12/IL-23 (p40) (ELISA Std.)

  • Recombinant Mouse IL-12 (p70) (ELISA Std.)

  • Recombinant Human IL-1β (ELISA Std.)

  • Human TruStain FcX™ (Fc Receptor Blocking Solution)

  • Recombinant Mouse IL-12 (p70) (carrier-free)

  • Recombinant Mouse IL-17F (carrier-free)

  • Recombinant Human IL-12 (p70) (carrier-free)

  • Recombinant Human EGF (Animal-Free)

  • Recombinant Human IFN-γ (Animal-Free)

  • LEGEND MAX™ Human IL-12 (p70) ELISA Kit

  • LEGEND MAX™ Human MRP8/14 (Calprotectin) ELISA Kit

  • Recombinant Human TWEAK (CD255) (carrier-free)

  • Recombinant Mouse IL-15 (carrier-free)

  • Recombinant Human IL-4 (carrier-free)

  • LEGEND MAX™ Human FGL2 ELISA Kit

  • LEGEND MAX™ Human IL-13 ELISA Kit

  • LEGEND MAX™ Human IL-12/IL-23 (p40) ELISA Kit

  • LEGEND MAX™ Mouse IL-17F ELISA Kit

  • LEGEND MAX™ Mouse IL-33 ELISA Kit

  • Recombinant Human IL-23 (carrier-free)

  • Recombinant Human IL-8 (carrier-free)

  • Recombinant Human IL-2 (carrier-free)

  • Recombinant Mouse IL-27 (carrier-free)

  • Recombinant Mouse IL-12 p40 Homodimer (carrier-free)

  • ELISA MAX™ Deluxe Set Mouse IL-22

  • DyLight™ 488 Streptavidin

  • DyLight™ 649 Streptavidin

  • LEGEND MAX™ Rat IL-6 ELISA Kit

  • Recombinant Mouse EGF (Animal-Free)

  • Recombinant Mouse FGF-basic (Animal-Free)

  • Recombinant Mouse G-CSF (Animal-Free)

  • Recombinant Human VEGF-165 (Animal-Free)

  • Recombinant Human TNF-α (Animal-Free)

  • Recombinant Human TRANCE (RANKL) (Animal-Free)

  • Recombinant Human IL-27 (carrier-free)

  • LEGEND MAX™ Human IL-1β ELISA Kit

  • Recombinant Mouse IL-34 (carrier-free)

  • Recombinant Human TGF-α (Animal-Free)

  • Recombinant Human Thrombopoietin (TPO) (Animal-Free)

  • Recombinant Human GM-CSF (Animal-Free)

  • LEGEND MAX™ Mouse IL-17A/F ELISA Kit

  • LEGEND MAX™ Rat IL-17A ELISA Kit

  • Recombinant Human Oncostatin M (Animal-Free)

  • Recombinant Human PDGF-AA (Animal-Free)

  • ELISA MAX™ Deluxe Set Rat TNF-a

  • ELISA MAX™ Deluxe Set Rat IL-17A

  • Brilliant Violet 421™ Streptavidin

  • Recombinant Mouse TACI-Fc Chimera (carrier-free)

  • FOXP3 Perm Buffer (10x)

  • Recombinant Mouse IL-7 (carrier-free)

  • Recombinant Human IL-34 (carrier free)

  • Recombinant Human M-CSF (Animal-Free)

  • Recombinant Human KGF (FGF-7) (Animal-Free)

  • Recombinant Human LIF (Animal-Free)

  • RBC Lysis/Fixation Solution (10X)

  • Recombinant Mouse CXCL9 (MIG) (carrier-free)

  • LEGEND MAX™ Rat IFN-γ ELISA Kit

  • LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit

  • LEGEND MAX™ Human Active MIF ELISA Kit

  • LEGEND MAX™ Human IL-17A/F ELISA Kit

  • Brilliant Violet 570™ Streptavidin