Purified anti-AICD Antibody (Previously Covance catalog# SIG-39148)

Pricing & Availability
Clone
Poly8119 (See other available formats)
Regulatory Status
RUO
Other Names
Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9148-005
Covance Catalog# SIG-39148
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly8119_PURE_AICD_Antibody_071621.png
Direct ELISA of purified anti-AICD antibody (clone Poly8119) binding to plate-immobilized recombinant CTF-50 (blue, positive control) and CTF-57 (red, negative control) proteins. ELISA was performed by coating wells with 0.5 µg/mL of recombinant proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Donkey anti-rabbit IgG antibody (Cat. No. 405405). TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) Substrate solution was used as the detection system.
  • Poly8119_PURE_AICD_Antibody_071621.png
    Direct ELISA of purified anti-AICD antibody (clone Poly8119) binding to plate-immobilized recombinant CTF-50 (blue, positive control) and CTF-57 (red, negative control) proteins. ELISA was performed by coating wells with 0.5 µg/mL of recombinant proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Donkey anti-rabbit IgG antibody (Cat. No. 405405). TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) Substrate solution was used as the detection system.
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811901 50 µg 360,00€
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Description

APP intracellular domain (AICD) is a peptide fragment that results from intramembraneous proteolysis of the amyloid precursor protein (APP). APP is a single-pass transmembrane protein that is involved in the pathophysiology of Alzheimer's disease (AD). APP is cleaved by gamma-secretase that releases the AICD into the cytoplasm. Soon after its release from the membrane, AICD is rapidly degraded in the cytoplasm partly by insulin degrading enzymes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e. add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ELISA
ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.01 - 1 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

 

Application Notes

This antibody is specific for AICD (APP intracellular domain), which is a peptide fragment that results from the cleavage of C83 or C99 at the epsilon site. It does not react with the C57 or C59 γ-secretase cleavage products of APP.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Kaori K, et al. 2012. Invest Opththalmol Vis Sci. 53:7675-83. (WB)
  2. Stieren ES, et al. 2011. J Biol Chem. 286:35689-98. (WB)
  3. Tampellini D, et al. 2011. J Neurosci. 31:15384-90. (IF) PubMed
Product Citations
  1. Tampellini D, et al. 2011. J Neurosci. 31:15384-15390. PubMed
  2. Hicks DA, et al. 2020. Bioscience Reports. 40(4):. PubMed
RRID
AB_2564761 (BioLegend Cat. No. 811901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about AICD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 01.25.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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