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PE anti-human CD239/B-CAM Recombinant Antibody

Pricing & Availability
Clone
QA20A43 (See other available formats)
Regulatory Status
RUO
Other Names
Lutheran antigen. Basal Cell Adhesion Molecule (B-CAM, Lutheran blood group). B-CAM cell surface glycoprotein
Isotype
Mouse IgG2b, κ
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Product Citations
publications
QA20A43_PE_CD239_Antibody_1_022823
Human peripheral red blood cells were stained with anti-human CD239/B-CAM recombinant (clone QA20A43) PE (left) or mouse IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD235 APC.
  • QA20A43_PE_CD239_Antibody_1_022823
    Human peripheral red blood cells were stained with anti-human CD239/B-CAM recombinant (clone QA20A43) PE (left) or mouse IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD235 APC.
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378803 25 tests 146€
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378804 100 tests 304€
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Description

QA20A43 recognizes human CD239, also known as Lutheran antigen or Basal Cell Adhesion Molecule (B-CAM). Human CD239 is a type I transmembrane glycoprotein that is encoded by B-CAM which belongs to the Ig gene superfamily. Full-length BCAM (CD239) is comprised of an extracellular region with two N-terminal V-type Ig-like domains followed by three C2-type Ig-like domains, a transmembrane region, and cytoplasmic domain of 59 amino acid residues. CD239 is expressed on erythrocytes in the peripheral blood and on the basal layer of vascular endothelial cells or some epithelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA20A43 can completely block the binding of positive clone to target cells.

RRID
AB_2936741 (BioLegend Cat. No. 378803)
AB_2936741 (BioLegend Cat. No. 378804)

Antigen Details

Structure
~85 kD single pass type I membrane glycoprotein
Distribution

Erythrocytes in the peripheral blood and on the basal layer of vascular endothelial cells or some epithelial cells

Function
CD239 is upregulated upon malignant transformation of some cell types including those found in ovarian carcinomas and plays a role in epithelial cell cancer and in the vaso-occlusion of red blood cells in sickle cell disease.
Ligand/Receptor
CD239 is a receptor for the extracellular matrix protein, laminin
Cell Type
Antigen-presenting cells, Endothelial cells, Epithelial cells, Erythrocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Soluble Receptors
Antigen References
  1. El Nemer W, et al. 1998. J Biol Chem. 273:16686-93. 
  2. Parsons SF, et al. 1997. Blood. 89:4219-25.

 

Gene ID
4059 View all products for this Gene ID
UniProt
View information about CD239/B-CAM on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.08.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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