FITC anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2
C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE
  • 17A2
    C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE
Compare all formats See FITC spectral data
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100203 50 µg 27€
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100204 500 µg 106€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  2. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  3. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  4. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  5. Wang X, et al. 2018. Mol Med Rep. 17:1660. PubMed
  6. Jia H, et al. 2018. Int J Oncol. 53:949. PubMed
  7. Solanki A, et al. 2018. Development. 145. PubMed
  8. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  9. Lee JS et al. 2018. Cell. 174(6):1559-1570 . PubMed
  10. Xu X, et al. 2018. Cell. 175:1336. PubMed
  11. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  12. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  13. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  14. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  15. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  16. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  17. Shields BD, et al. 2019. Cancer Res. 79:1113. PubMed
  18. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  19. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  20. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  21. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  22. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  23. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  24. Chen X, et al. 2019. Med Sci Monit. 25:5389. PubMed
  25. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  26. Li W, et al. 2019. Nat Commun. 10:3349. PubMed
  27. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  28. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  29. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  30. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  31. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
  32. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
  33. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  34. Roy S, et al. 2019. Front Immunol. 2.404166667. PubMed
  35. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  36. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  37. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  38. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  39. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  40. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  41. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  42. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  43. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  44. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  45. Pan X, et al. 2019. BMC Complement Altern Med. 19:163. PubMed
  46. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  47. Martínez-Vicente P, et al. 2019. PLoS Pathog. 15:e1007658. PubMed
  48. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  49. Lin W, et al. 2020. Theranostics. 10:4871. PubMed
  50. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  51. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  52. Sreekumar PG, et al. 2018. J Control Release. 283:94. PubMed
  53. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  54. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  55. Tur J, et al. 2020. Cell Reports. 32(8):108079. PubMed
  56. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  57. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  58. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  59. Lesmeister M, et al. 2005. Reprod Biol Endocrinol. 29:74. PubMed
  60. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  61. Wu X, et al. 2012. Biochem Pharmacol. 84:1164. PubMed
  62. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  63. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
  64. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  65. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
  66. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  67. White C, et al. 2014. J Immunol. 193:5933. PubMed
  68. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  69. Huang B, et al. 2015. PLoS One. 10: 0134715. PubMed
  70. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  71. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  72. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  73. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  74. Mingozzi F, et al. 2016. EMBO Mol Med. 8: 1039 - 1051. PubMed
  75. Chen Q, et al. 2016. Nat Commun. 7:13193. PubMed
  76. Akhter N, et al. 2016. J Biol Chem. 291: 23672 - 23680. PubMed
  77. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  78. Zan H, et al. 2017. Nat Commun. 8:14244. PubMed
  79. Wu L, et al. 2020. Cell Rep. 33:108327. PubMed
  80. Zheng DW, et al. 2020. Nat Commun. 3.865972222. PubMed
  81. Zheng L, et al. 2020. Front Oncol. 10:531131. PubMed
  82. Campolo M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  83. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  84. Bao H, et al. 2020. Mol Med Rep. 4.675. PubMed
  85. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  86. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  87. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  88. Dai L, et al. 2020. Cell. 182(3):722-733.e11. PubMed
  89. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  90. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  91. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  92. Li J, et al. 2021. eLife. 10:00. PubMed
  93. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  94. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  95. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  96. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  97. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  98. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  99. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  100. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  101. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
RRID
AB_312660 (BioLegend Cat. No. 100203)
AB_312661 (BioLegend Cat. No. 100204)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC, ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F, FC, 3D IHC, SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

  • Spark Red™ 718 anti-mouse CD3

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