Recombinant Human WNT-7a (carrier-free)

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Other Names
Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A)
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597901 3 µg 64€
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597902 15 µg 148€
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Description

The WNT protein family has a highly conserved cystein-rich structure, associated with the extracellular matrix (ECM) with high affinity through binding to heparin sulfate glycoproteins. They are carried intercellularly by lipoproteins and mediate signaling activity by binding to transmembrane Frizzled receptors and low-density-related proteins (LRP). They elicit a variety of responses through canonical or non-canonical (PCP or Ca++) pathways. There are two subtypes of WNT-7 that have been identified, WNT-7a and WNT-7b.

WNT-7a is a secreted cell surface signaling protein that controls a set of pathways that act on cell fate determination and tissue patterning during embryogenesis. It is known that WNT-7a can modulate the development of the uterus, cerebellum and limbs, while WNT-7b is important in lung and placental development. The human amino acid sequence of WNT-7a shares high similarity (99.4%) with mouse WNT-7a. Signals transduced through WNT-7a and its receptor are found to be critical for female organ development, maintaining levels of female sex hormones and the development of oviduct and uterus. Receptors for WNT-7a include frizzled-5, frizzled-7 and frizzled-10. WNT-7a binds to frizzled-7 to induce signaling through SKT/ mTOR pathway in order to further modulate myofibers in a G-protein dependent PI3K pathway.

During muscle cell regeneration, WNT-7a in the ECM along with transiently raised fibronectin levels in the stem cell niche can bind the frizzled-7/Sdc4 receptor complex and synergistically induce symmetric divisions of satellite stem cells. Also, in neurogenesis and chondrogenesis, WNT-7a contributes both in regulating and maintaining the level of lineage differentiation factors required to support apoptosis / cell death through binding of different receptors and interactions with ECM proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human WNT-7a, amino acids Leu32-Lys349 (Accession# NM_004625.3), was expressed in HEK293 cells
Molecular Mass
The 318 amino acids recombinant protein has a predicted molecular mass 35.5 kD. The predicted N-terminal amino acid is Leu.
Purity
>80% by SDS-PAGE gel and HPLC analyses.
Formulation
Lyophilized
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of ≤0.2mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The ED50 was determined by its ability to inhibit WNT-3a induced alkaline phosphatase production in MC3T3-E1 cells. The estimated ED50 is 40-60 ng/ml, corresponding to a specific activity of 1.67 - 2.5 x 104 units/mg.
Application

Bioassay

Antigen Details

Structure
Cytokine
Distribution
WNT-7a is expressed in the placenta, kidney, testis, uterus, fetal lung, fetal and adult brain.
Function
WNT-7a is important for dorsal ectoderm tissue development and regulation.
Interaction
WNT-7a interacts broadly with tissue derived neuronal cells, epithelial cells, and endothelial cells.
Ligand/Receptor
Frizzled receptors, LRP co-receptors.
Bioactivity
WNT-7a mediates regulation of body axis patterning, cell lineage determination, and cell proliferation and migration during embryogenesis.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Feinberg RN, et al. 1983. J. Exp. Zool. 226:391.
2. Dealy CN, et al. 1993. Mech. Dev. 43:175.
3. von Maltzahn J, et al. 2011. Nat. Cell Biol. 14:186. 
4. Parr BA, et al. 1998. Nature 395:707.

Gene ID
7476 View all products for this Gene ID
UniProt
View information about WNT-7a on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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