Recombinant Human PLGF-2 (carrier-free)

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Other Names
Placenta Growth Factor 2, PLGF2,
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Human_PLGF-2_RECOM_CF_060517
When human PLGF-2 is immobilized at 0.5 µg/mL, human VEGF-R1 binds in a dose dependent manner with EC50 of 0.1 - 0.4 µg/mL.
  • Human_PLGF-2_RECOM_CF_060517
    When human PLGF-2 is immobilized at 0.5 µg/mL, human VEGF-R1 binds in a dose dependent manner with EC50 of 0.1 - 0.4 µg/mL.
  • Human_PLGF-2_RECOM_CF_Stability_103117
    Human PLGF-2 was aliquoted in PBS at 0.2 mg/ml and was treated as followed: one aliquot was kept at 4°C (control), another was freeze-thawed four times (4x freeze-thaws), one incubated for seven days at room temperature (RT), and another incubated at 37°C for one week. After this procedure, the samples were tested for their ability to bind human VEGF-R1 in a functional ELISA.
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765202 10 µg 72€
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765204 25 µg 152€
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Description

Placenta growth factor (PLGF) was initially cloned from a term placenta cDNA library.  It is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. As a result of alternative splicing, four isoforms for PLGF has been described, PLGF-1 (PLGF-131), PLGF-2 (PLGF-152), PLGF-3 (PLGF-203), and PLGF-4 (PLGF-224).  While PLGF isoforms are present in most placental tissues, PLGF-2 is specific to early placenta and only PLGF-1 is found in the colon and mammary carcinomas.  The PLGFs are secreted, but PLGF-2 appears to remain cell attached unless released by heparin.  PLGF-2 binds to heparin with high affinity, whereas PLGF-1, PLGF-3, and PLGF-4 do not bind heparin.  PLGF-2 is the only isoform found in mouse. PLGF binds to VEGF-R1 and neuropilins, but not to VEGF-R2.  PLGF induces monocyte activation, migration, and production of inflammatory cytokines.  PLGF plays a key role in the maternal vascular function during pregnancy. Circulating PLGF increases during pregnancy, reaching a peak in mid‑gestation. Low levels of PLGF have been associated with patients experiencing preeclampsia.  High expression of PLGF has been associated to colorectal cancer (CRC), and its expression in CRC tissues correlates with high levels of MMP-9 and VEGF-R1. PLGF has been shown to increase tumor cell migration in lung cancer, leukemia, and melanoma cells.  In colorectal cancer, PLGF induces tumor cell migration and invasion through upregulation of MMP9.  PLGF is expressed in the majority of medulloblastomas, and high expression of PLGF receptor neuropilin 1 (Nrp1) correlates with poor survival in patients. In a mouse model, PLGF/Nrp1 blockade provided direct antitumor effects in vivo, including medulloblastoma regression, decreased metastasis, and increased survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human PLGF-2, amino acids Leu19-Arg170 (Accession # NP_002623) was expressed in CHO cells.
Molecular Mass
The 152 amino acid recombinant protein has a predicted molecular mass of approximately 17.3 kDa. The DTT-reduced protein migrates at approximately 25 kDa, and non-reduced proteins migrate at approximately 50 kDa by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 1.0 EU per µg protein as determined by the LAL method.
Concentration
10 - 100 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When human PLGF-2 is immobilized at 0.5 µg/mL, human VEGF-R1 binds in a dose dependent manner with EC50 of 0.1 - 0.4 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Expressed in vascular endothelial cells, chondrocytes, placental syncytiotrophoblasts, stromal cells of mesenchymal origin, smooth muscle cells, hematopoietic and mesenchymal stem cells, activated monocytes, and leukemic cells of lymphoid and myeloid lineage.

Function
PLGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PLGF.
Interaction
Endothelial cells
Ligand/Receptor
VEGF-R1, Neuropilin-1
Bioactivity
Measured by the ability of immobilized protein to support the adhesion of C6 glioma cells.
Biology Area
Cell Biology, Signal Transduction, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Maglione D, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9267.
2. Carmeliet P, et al. 2001. Nat. Med. 7:575.
3. Casalou C, et al. 2007. Leukemia 21:1590.
4. Marcellini M, et al. 2006. Am. J. Pathol. 169:643.
5. Yao J, et al. 2011. Proc. Natl. Acad. Sci. USA 108:11590.
6. Cai J, et al. 2011. PLoS One 6:e18076.
7. Sibiude J, et al. 2012. PLoS One 7:e50208.
8. Snuderl M, et al. 2013 Cell 152:1065.

Gene ID
5228 View all products for this Gene ID
UniProt
View information about PLGF-2 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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