Recombinant Mouse IL-2 (carrier-free)

Pricing & Availability
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
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IL-2_Mouse_Recombinant_Protein_BA_032913
HT-2 cell proliferation induced by mouse IL-2.
  • IL-2_Mouse_Recombinant_Protein_BA_032913
    HT-2 cell proliferation induced by mouse IL-2.
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Cat # Size Price Save
575402 10 µg ¥14,400
575404 25 µg ¥32,400
575406 100 µg ¥72,000
575408 500 µg ¥225,000
Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes.

Product Details
Technical data sheet

Product Details

Source
Mouse IL-2, amino acids Ala21-Gln169 (Accession # NM_008366) was expressed in E. coli.
Molecular Mass
The 150 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17,231 Da. The DTT-reduced protein and non-reduced protein migrates at approximately 18kDa by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 5 mM NaH2PO4, 5 mM citric acid, 350 mM NaCl, 1 mM TCEP, pH 4.0.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.2 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 5.0 x106 units/mg, as determined by the dose dependent stimulation of CTLL2 cell proliferation.

The specific activity of recombinant mouse IL-2 is approximately 9.07 x 104 IU/µg when compared against the Non WHO Reference Material for Murine Interleukin-2 (NIBSC code: 93/566) as determined by the dose dependent stimulation of CTLL2 cell proliferation.

ED50 = 0.4 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.5 x106 units/mg, as determined by the dose dependent stimulation of HT-2 cell proliferation.

For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…

Application References

(PubMed link indicates BioLegend citation)
  1. Detanico T, et al. 2011.J. Immunol. 187:82. PubMed
  2. Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
  3. Alcaide P, et al. 2012. J. Immunol. 188:1421. PubMed
  4. Williams JW, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:693. PubMed
Product Citations
  1. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  2. Liu B, et al. 2019. Nat Commun. 10:354. PubMed
  3. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  4. Jiang P, et al. 2018. Nat Med. 24:1550. PubMed
  5. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  6. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  7. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  8. Han S, et al. 2020. Oncoimmunology. 9:1737368. PubMed
  9. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  10. Loo CS, et al. 2020. Immunity. 53:143. PubMed
  11. Gomez–Lopez N, et al. 2019. J Immunol. 203:1793. PubMed
  12. Ruscetti M, et al. 2020. Cell. 181:424. PubMed
  13. Detanico T, et al. 2011. J Immunol. 187:82. PubMed
  14. Jacque E, et al. 2015. J Exp Med. 212:883. PubMed
  15. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  16. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  17. Bao K, et al. 2016. J Immunol. 197(11):4371-4381. PubMed

Antigen Details

Structure
Cytokine
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines
Regulation
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Gene ID
16183 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 5    Revision Date: 05/04/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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