- Clone
- CUB1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HLDA8
- Other Names
- CDCP1, CUB domain containing protein 1, SIMA135
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![CUB1_Purified_042307 CUB1_Purified_042307](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/CUB1_Purified_042307.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HT-29 cells (human colon carcinoma) were stained with purified anti-human CD318 (clone CUB1) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
The CUB1 antibody recognizes CD318 also known as CDCP1, CUB domain containing protein 1, and SIMA135. CD318 is a transmembrane protein containing three CUB domains with a predicted molecular weight of approximately 93 kD. Alternately spliced isoforms have also been reported. CD318 is a marker for immature hematopoietic progenitor cell subsets and is also expressed in mesenchymal stem cells and neural progenitor cell populations. This marker is also widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small intestine. CD318 is overexpressed in colon and lung tumors and expression level has been shown to correlate with metastatic ability of carcinomas. CD318 interacts with plasminogen, matriptase, and c-Src (phosphorylates residue Pyr734). The function of CD318 is not well understood although it is thought to be involved in early hematopoiesis. The CUB1 antibody has been shown to be useful for flow cytometry.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NIH-3T3 cells transfected with human CDCP1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Bühring HJ, et al. 2004. Stem Cells 22:334-343.
- Product Citations
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- RRID
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AB_756052 (BioLegend Cat. No. 324002)
Antigen Details
- Structure
- Transmembrane protein containing three CUB domains, predicted molecular weight approximately 93 kD. Alternately spliced isoforms have been reported
- Distribution
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Marker for immature hematopoietic progenitor cell subsets; also expressed in mesenchymal stem cells and neural progenitor cell populations. Widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small
- Function
- Unknown, thought to be involved on early hematopoiesis
- Interaction
- Plasminogen, matriptase, c-Src
- Modification
- Phosphorylation (Y734) by c-Src; regulated by adhesion or plasmin in epithelial cells
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Brown TA, et al. 2004. J. Biol. Chem. 279:14772.
2. Conze T, et al. 2003. Ann. N.Y. Acad. Sci. 996:222.
3. Hooper JD, et al. 2003. Oncogene 22:1783. - Gene ID
- 64866 View all products for this Gene ID
- UniProt
- View information about CD318 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD318 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD318 (CDCP1) | CUB1 | FC |
APC anti-human CD318 (CDCP1) | CUB1 | FC |
FITC anti-human CD318 (CDCP1) | CUB1 | FC |
Purified anti-human CD318 (CDCP1) | CUB1 | FC |
TotalSeq™-A1254 anti-human CD318 (CDCP1) | CUB1 | PG |
TotalSeq™-B1254 anti-human CD318 (CDCP1) | CUB1 | PG |
TotalSeq™-C1254 anti-human CD318 (CDCP1) | CUB1 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD318 (CDCP1)
HT-29 cells (human colon carcinoma) were stained with PE ant... -
APC anti-human CD318 (CDCP1)
HT-29 cells (human colon carcinoma) were stained with APC an... -
FITC anti-human CD318 (CDCP1)
HT-29 cells (human colon carcinoma) were stained with FITC a... -
Purified anti-human CD318 (CDCP1)
HT-29 cells (human colon carcinoma) were stained with purifi... -
TotalSeq™-A1254 anti-human CD318 (CDCP1)
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TotalSeq™-B1254 anti-human CD318 (CDCP1)
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TotalSeq™-C1254 anti-human CD318 (CDCP1)
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