Biotin anti-human CCL19 Antibody

Pricing & Availability
Clone
Poly5384 (See other available formats)
Regulatory Status
RUO
Other Names
EBI1 ligand chemokine, ELC, macrophage inflammatory protein-3-beta
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5384_Biotin_-CCL19_-Direct-ELISA_Antibody_052323
Direct ELISA showing biotin anti-human CCL19 (clone Poly5384) bound to plate-immobilized recombinant human CCL19 (MIP-3β) protein (Cat. Nos. 582102, 582104, and 582106). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • Poly5384_Biotin_-CCL19_-Direct-ELISA_Antibody_052323
    Direct ELISA showing biotin anti-human CCL19 (clone Poly5384) bound to plate-immobilized recombinant human CCL19 (MIP-3β) protein (Cat. Nos. 582102, 582104, and 582106). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
Cat # Size Price Save
538403 50 µg ¥58,300
Description

CCL19 is a CC chemokine expressed in lymph nodes. CCL19 is only 32% identical to CCL21 at the amino acid level; nevertheless, these chemokines bind to the same receptor, CCR7. CCL19 is not expressed in high endothelial venules (HEV) as CCL21, but CCL19 is transcytosed in HEV. It has been described that CCL19 binds to CCX-CKR and CRAM-B receptors. These two receptors are members of the non-classical chemokine receptor family, which also includes D6, DARC, and CXCR7. It has been suggested that CCX-CKR is a scavenging receptor for CCL19, and CRAM-B receptor might be responsible for the transcytosis in HEV for CCL19. In addition, CRAM recycles constitutively and can internalize CCL19 within a few minutes, suggesting it may play a role in the regulation of immune responses as well as homing processes. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Immunogen
Human Recombinant CCL19 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly5384) could function as a detection antibody when paired with Ultra-LEAF™ purified anti-human CCL19 antibody (Cat Nos. 612803 and 612804) (clone A15093C) as the capture antibody and using Recombinant Human CCL19 (MIP-3β) protein (Cat. Nos. 582102, 582104, and 582106) as the assay standard.

RRID
AB_3083212 (BioLegend Cat. No. 538403)

Antigen Details

Structure
Chemokine
Distribution

CCL19 is expressed in the T cell zone of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL19 is expressed by endothelial cells in lymphatic venules.

Function
CCL19 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL19 and CCL21 inhibit apoptosis in DCs.
Interaction
Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, antigenengaged B cells, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
Ligand/Receptor
CCR7 (CD197)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Rossi DL, et al. 1997. J Immunol. 158:1033-6.
  2. Sánchez-Sánchez N, et al. 2004. Blood. 104:619-25.
  3. Comerford I, et al. 2006. Eur. J Immunol. 36:1904-16.
  4. Leick M, et al. 2009. Immunology. 129:536.
  5. Le Nouën C, et al. 2011. PLoS Pathog. 7:e1002105.
Gene ID
6363 View all products for this Gene ID
UniProt
View information about CCL19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CCL19 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CCL19 Poly5384 Direct ELISA
Biotin anti-human CCL19 Poly5384 Direct ELISA
Go To Top Version: 1    Revision Date: 06/16/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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