Recombinant Human APRIL/TNFSF13 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
A Proliferation Inducing Ligand (APRIL), TNFSF13, CD256, TALL2, TRDL1
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Recom_ Human APRIL-TNFSF13_1_CF
Recombinant human TNFSF13 induces the proliferation of mouse B cells in a dose-dependent manner. The ED50 for this effect is 0.1 – 0.5 ng/mL.
  • Recom_ Human APRIL-TNFSF13_1_CF
    Recombinant human TNFSF13 induces the proliferation of mouse B cells in a dose-dependent manner. The ED50 for this effect is 0.1 – 0.5 ng/mL.
  • Recom_ Human APRIL-TNFSF13_2_CF
    Stability Testing for Recombinant human TNFSF13. Recombinant human TNFSF13 was aliquoted in 20mM Tris, 500 mM NaCl, pH 8.0 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce the proliferation of mouse B cells in a dose-dependent manner. The ED50 for this effect is 0.1 – 0.5 ng/mL.
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568004 25 µg 464€
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568006 100 µg 796€
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Description

APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the TNF superfamily of ligands and stimulates cell proliferation. APRIL shares 30% amino acid sequence identity with BAFF. APRIL is cleaved intracellularly by furin and is believed to exist mainly in a secreted, soluble form. As with most other TNF family members, APRIL exists as a functional homotrimer (total MW = 90 kD). In conjunction with BAFF, APRIL binds cell-surface receptors, BCMA and TACI to exert T cell-dependent and -independent antibody secretion and B cell differentiation. In addition, APRIL possesses a heparan sulfate proteoglycan binding domain and has been demonstrated to bind to proteoglycans on the cell surface in vitro and in situ.

APRIL is a potential biomarker and is most well-known for tumor proliferation effects. For certain cancers, APRIL levels are elevated in serum while for other cancers, higher tissue expression levels of APRIL have been observed. In addition, APRIL is a potential biomarker for certain autoimmune diseases. Recently, recombinant TACI has been brought to clinical trials as a neutralization drug against APRIL and BAFF for the treatment of systemic lupus erythematosus (SLE). Furthermore, besides forming homotrimers, APRIL can also form functional heterotrimers with BAFF. Although the stoichiometric relationship of the protomeric units is still unclear, it appears that these heterotrimers are significant in that they are elevated in the serum of certain autoimmune patients. APRIL also forms an endogenous chimeric protein with another related protein, TWEAK, effectively called TWE-PRIL. It is believed that TWE-PRIL is membrane bound and consists of the N-terminus of TWEAK with its transmembrane domain, and the C-terminus of APRIL with its receptor binding domain.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNFSF13, amino acid Ala105-Leu250 (Accession# O75888.1), with N-terminal HA tag, followed with GCN4tri and a 3(GGGGS) linker, was expressed in CHO cells.
Molecular Mass
The 202 amino acid recombinant protein has a predicted molecular mass of approximately 22.19 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 20 – 40 kD by SDS-PAGE. The predicted N-terminal amino acid is Tyr.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
20 mM Tris, 500 mM NaCl, pH 8.0
Endotoxin Level
Less than 0.1 EU per µg of cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human TNFSF13 induces mouse B cell proliferation in a dose-dependent manner. The ED50 is 0.1 - 0.5 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Trimer
Distribution

T cells, B cells, dendritic cells, monocytes, and macrophages

Function
Induces proliferation of T and B cells, promotes IgA class switching and downregulates T-cell dependent antibody responses, regulates the growth of a variety of tumor cells
Interaction
T cells, B cells, cancer cells
Ligand/Receptor
TNFRSF13b (TACI), TNFRSF17 (BCMA)
Bioactivity
ED50 = 0.1 – 0.5 ng/mL as determined by the dose-dependent stimulation of mouse B cell proliferation.
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Cytokines/Chemokines, Soluble Receptors
Antigen References
  1. Hymowitz S, et al. 2005. J Biol Chem. 280:7218-27.
  2. Ingold K, et al. 2005. J Exp Med. 201:1375-83.
  3. Good K, et al. 2009. J Immunol. 182:890-901.
  4. Bossen C, et al. 2008. Blood. 111:1004-12.
  5. Bossen C, et al. 2006. J Biol Chem. 281:13964-71.
  6. Pradet-Balade B, et al. 2002. EMBO J. 21:5711-20.
  7. Hahne M, et al. 1998. J Exp Med. 188:1185-90.
  8. Medema JP, et al. 2003. Cell Death Differ. 10:1121-5.
  9. Planelles L, et al. 2007. Haematologica. 92:1284-5.
  10. Dillon SR, et al. 2010. Arthritis Res. Ther 12:R48.
  11. Wallweber H, et al. 2004. J Mol Biol. 343:283-90.
  12. Chen Y, et al 2011. PLoS ONE. 6:e22202.
  13. Roschke V, et al. 2002. J Immunol. 169:4314-21.
Gene ID
8741 View all products for this Gene ID
UniProt
View information about APRIL/TNFSF13 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 01/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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