Ultra-LEAF™ Purified anti-mouse CD16 Antibody

Pricing & Availability
Clone
W20015B (See other available formats)
Regulatory Status
RUO
Other Names
Fc-Gamma Receptor III-2 (CD16), Immunoglobulin G Fc Receptor III, Fc Fragment Of IgG, Low Affinity III, Receptor For, Low Affinity Immunoglobulin Gamma Receptor III-A Fc Fragment, Fc Fragment Of IgG Receptor IIIa, Fc-Gamma RIII-Alpha
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W20015B_ULEAF_CD16_012822
Biotin anti-mouse IgG1 (Cat. No. 406604) (black circles) binds to immobilized recombinant mouse FcγRIII (CD16) (Cat. No. 790104). Ultra-LEAF™ purified anti-mouse CD16 (clone W20015B) (purple squares) inhibits the binding in a dose-dependent manner whereas the purified rat IgG2b, λ isotype control (Cat. No. 403801) (gray triangles) does not have the effect. This antibody blocks the binding of 11 µg/mL biotin anti-mouse IgG1 to 5 µg/mL immobilized recombinant mouse FcγRIII (CD16). ND50 range: 0.02 - 0.15 µg/mL
  • W20015B_ULEAF_CD16_012822
    Biotin anti-mouse IgG1 (Cat. No. 406604) (black circles) binds to immobilized recombinant mouse FcγRIII (CD16) (Cat. No. 790104). Ultra-LEAF™ purified anti-mouse CD16 (clone W20015B) (purple squares) inhibits the binding in a dose-dependent manner whereas the purified rat IgG2b, λ isotype control (Cat. No. 403801) (gray triangles) does not have the effect. This antibody blocks the binding of 11 µg/mL biotin anti-mouse IgG1 to 5 µg/mL immobilized recombinant mouse FcγRIII (CD16). ND50 range: 0.02 - 0.15 µg/mL
Cat # Size Price Quantity Check Availability Save
945803 100 µg 287€
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945804 1 mg 964€
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Description

CD16, also known as FcγRIII, is a low affinity IgG Fc receptor for a broad range of different isotypes. It primarily exists as two distinct forms: FcγRIIIA/CD16a and FcγRIIIB/CD16b, which are encoded by two nearly identical genes in humans. The expression of the two isoforms is tissue-specific. FcγRIIIA is a 50-65 kD type I transmembrane protein that is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. FcγRIIIB is a 48 kD GPI-anchored protein. Its extracellular domain is over 95% homologous to that of FcγRIIIA, and it is expressed specifically on neutrophils. FcγRIIIA can bind aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). Several studies indicated that the post-translational modifications of FcγRIIIA or IgG regulate FcγRIIIA-mediated functions. The glycosylation of FcγRIIIA is mandatory for high affinity binding to Fc. Additionally, lack of fucosylation of Fc glycans dramatically enhance ADCC by improving the binding of FcγRIIIA and IgG. It has been reported that the polymorphism (F176V) showed a greater binding capacity to IgG, leading to increasing antibody-dependent NK cell cytotoxicity and susceptibility to autoimmune diseases. The missense mutation (L66H) causes immunodeficiency 20 disease (IMD20). FcγRIIIA associates with γ and zeta chains of T-cell antigen receptor complex (CD3:TCR) and FcεRIγ on human NK cells, suggesting involvement of distinct signal-transducing function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD16 (Fcγ RIII)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 11 µg/mL biotin anti-mouse IgG1 antibody (Cat. No. 406604) to 5 µg/mL immobilized recombinant mouse FcγRIII (CD16) (Cat. No. 790104). ND50 range: 0.02 - 0.15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2910506 (BioLegend Cat. No. 945803)
AB_2910506 (BioLegend Cat. No. 945804)

Antigen Details

Structure
Immunoglobulin family
Distribution

NK cells, activated monocytes, macrophages, neutrophils, subpopulation of T cells, immature thymocytes

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Interaction
FcγR subunit
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Bruhns P. 2012. Blood. 119:5640-9.
  2. Hirano M, et al. 2007. Nat Immunol. 8:762-71.
  3. Takai T. 2002. Nat Rev Immunol. 2:580-92.
  4. Jakus Z, et al. 2008. J Immunol. 180:618-29.
  5. Nimmerjahn F and Ravetch JV. 2005. Science. 310:1510-2.
  6. Hazenbos WL, et al. 1996. Immunity. 5:181-8.
  7. Hazenbos WL, et al. 1998. J Immunol. 161:3026-32.
  8. Watanabe N, et al. 1999. Blood. 94:3855.
Gene ID
14131 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-mouse CD16

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