Purified anti-p97/VCP Antibody

Pricing & Availability
Clone
4G9 (See other available formats)
Regulatory Status
RUO
Other Names
Transitional endoplasmic reticulum ATPase, p97, TERA
Isotype
Mouse IgG1, κ
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Product Citations
publications
4G9_WB_040910
Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
  • 4G9_WB_040910
    Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
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636801 25 µg 76,00€
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636802 100 µg 156,00€
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Description

p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human valosin containing protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C."
Application

WB - Quality tested
IP, ICC - Reported by the developer, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 mL antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed.
  2. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed.
  3. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed.
Product Citations
  1. Shchedrina V, et al. 2011. J Biol Chem. 286:42937. PubMed
  2. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed
  3. Yi L, et al. 2012. Hum Mol Genet. 2.120833333. PubMed
RRID
AB_2257136 (BioLegend Cat. No. 636801)
AB_2214641 (BioLegend Cat. No. 636802)

Antigen Details

Structure
Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction
VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP
Bioactivity
VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Neuroscience Cell Markers
Molecular Family
Endoplasmic Reticulum Markers
Antigen References

1. Ye Y, et al. 2001. Nature 414:652.

Gene ID
7415 View all products for this Gene ID
UniProt
View information about p97/VCP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/14/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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