- 11-5.2 (See other available formats)
- Regulatory Status
- Other Names
- MHC class II
- Mouse (BALB/c) IgG2b, κ
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- Product Citations
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The 11-5.2 antibody reacts with the α chain of the I-Ak MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2r haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation.Product Details
- Mouse k haplotype, Cross-reacts with H-2r haplotypes
- Antibody Type
- Host Species
- CKB mouse splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections.
This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).
(PubMed link indicates BioLegend citation)
- Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
- Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
- Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
- Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
- Ardehali A, et al. 1995. Circulation 92:450. (IHC)
- Turnquist HR, et al. 2007. J. Immunol. 178:7018.
- Zhang L, et al. 2011. Lupus. 20:667. PubMed.
- Product Citations
AB_313459 (BioLegend Cat. No. 110002)
- α chain of MHC class II
B cell and activated T cells, APCs of H-2b mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- Gene ID
- 14960 View all products for this Gene ID
- View information about I-Ak on UniProt.org
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