ELISA MAX™ Deluxe Set Human BAFF

Pricing & Availability
Regulatory Status
RUO
Other Names
BLys, TNFSF13B, B cell activating factor
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ELISA-MAX_Deluxe_Human_BAFF_051123
  • ELISA-MAX_Deluxe_Human_BAFF_051123
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449704 5 plates 395 CHF
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Description

BAFF (known as B Lymphocyte Stimulator) is a member of the Tumor Necrosis Factor Ligand Superfamily. The extracellular domain of BAFF shows high homology with the proliferation-inducing ligand (APRIL protein). BAFF acts mainly as a soluble trimer and binds to three receptors with decreasing affinity in the following order: BAFFR > TACI > BCMA. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, SLE, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin’s lymphoma (NHL).

BioLegend’s ELISA MAX™ Deluxe Set is designed for the accurate quantification of natural and recombinant human BAFF in serum, plasma, and cell culture supernatant.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human BAFF ELISA MAX™ Capture Antibody (200X)
  • Human BAFF ELISA MAX™ Detection Antibody (200X)
  • Human BAFF Standard Lyophilized
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Assay Diluent D

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
10.1 ± 7.1 pg/mL
Standard Range
31.25 - 2000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Epithelial cells, Lymphocytes, Monocytes, Neutrophils, Plasma cells, T cells
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
Molecular Family
Tumor Suppressors
Gene ID
NA
UniProt
View information about BAFF on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 05.11.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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