ELISA MAX™ Deluxe Set Human Amyloid Beta (1-42)

Pricing & Availability
Regulatory Status
RUO
Other Names
Human Aβ42, Amyloid-beta protein 42
Ave. Rating
Submit a Review
Product Citations
publications
ELISA-MAX_Deluxe_Human_Amyloid-Beta-1-42_060523
  • ELISA-MAX_Deluxe_Human_Amyloid-Beta-1-42_060523
Cat # Size Price Quantity Check Availability Save
448704 5 plates 395 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The Amyloid peptides are peptides of 36-43 amino acids which are produced through the post-translational cleavage of the Amyloid Precursor Protein by beta and gamma secretase. Although numerous different Amyloid-Beta species exist, the majority of the Amyloid-Beta peptides produced by post-translational modification consists of either Amyloid-Beta (1-40) (80-90%) and Amyloid-Beta (1-42) (5-10%). Both variants are major components of the extracellular plaques found in Alzheimer’s brain. However, Amyloid-Beta (1-42) is reported to be more prone to aggregation than the (1-40) variant despite differing in only 2 amino acids at the C terminus. Other studies that aimed at characterizing the difference in aggregation tendency between the two variants suggested that they have distinct 3D structures in solution.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for human Amyloid-Beta (1-42). This kit is specifically designed for the accurate quantitation of human Amyloid-Beta (1-42) in cerebrospinal fluid, serum and plasma. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Amyloid- Beta (1-42) Capture Antibody (200X)
  • Human Amyloid- Beta (1-42) Detection Antibody (200X)
  • Human Amyloid-Beta (1-42) Lyophilized Standard
  • Streptavidin-Polymer HRP (1,000X)
  • Coating Buffer B (5X)
  • Blocking Buffer A (2X)
  • ELISA Diluent 1 (20X)
  • Substrate Solution D

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.718 ± 0.190 pg/mL
Standard Range
3.125 - 200 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Neural Stem Cells, Neurons
Biology Area
Cell Death, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid, APP/Aβ Degradation
Gene ID
NA
UniProt
View information about Amyloid Beta 1-42 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 06.05.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account