- Regulatory Status
- Other Names
- LIF, CDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, interleukin 6 family cytokine
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|740605||100 tests (270 µL)||$100|
The LEGENDplex™ Human Hematopoietic Stem Cell Panel Mix and Match Beads are individual capture beads for 12-targets including:
740597 LEGENDplex™ Human IL-6 Capture Bead A4, 13X
740598 LEGENDplex™ Human FLT3L Capture Bead A5, 13X
740599 LEGENDplex™ Human GM-CSF Capture Bead A6, 13X
740601 LEGENDplex™ Human IL-3 Capture Bead A8, 13X
740602 LEGENDplex™ Human IL-34 Capture Bead A10, 13X
740603 LEGENDplex™ Human IL-11 Capture Bead B2, 13X
740604 LEGENDplex™ Human SCF Capture Bead B3, 13X
740605 LEGENDplex™ Human LIF Capture Bead B4, 13X
740606 LEGENDplex™ Human CXCL12 (SDF-1) Capture Bead B5, 13X
740607 LEGENDplex™ Human IL-15 Capture Bead B6, 13X
740608 LEGENDplex™ Human M-CSF Capture Bead B7, 13X
740609 LEGENDplex™ Human IL-7 Capture Bead B9, 13X
Selected items will be used together with:
740619 LEGENDplex™ Human Hematopoietic Stem Cell Panel Standard
740618 LEGENDplex™ Human Hematopoietic Stem Cell Panel Detection Antibodies
740620 LEGENDplex™ Buffer Set H
740377 LEGENDplex™ Filter Plate or 740379 LEGENDplex™ V-bottom Plate
The LEGENDplex™ Human Hematopoietic Stem Cell Panel (12-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 12 key targets involved in hematopoietic stem cell differentiation and lineage specific markers including CXCL12 (SDF-1), FLT3L, GM-CSF, IL-11, IL-15, IL-3, IL-34, IL-6, IL-7, LIF, M-CSF, and SCF. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.