Purified anti-mouse IFN-β Antibody

Pricing & Availability
Clone
Poly5192 (See other available formats)
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-G1 factor, IFN-b, IFN-beta
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
519202 100 µg $225
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Description

Interferon-β is a member of the type I interferon class. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα/β receptor (IFNAR), which is found on almost all cell types, and, via the JAK-STAT pathway, induce the expression of hundreds of IFN-stimulated genes (ISGs). While the function of many of these proteins remains to be elucidated, a hallmark of type I IFNs is their anti-viral activities, both general and specific against particular viruses. Type I IFNs also regulate cell growth and apoptosis, and exert various immunomodulatory and inflammatory effects. As such, they are also clinically relevant, both as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer.

Mouse IFN-β is a ~20 kD monomeric glycoprotein. It has 50% amino acid sequence homology with mouse IFN-α, its closest related protein, and 62% homology with human IFN-β. IFN-β is currently the therapeutic of choice for treating multiple sclerosis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IFN-β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: To measure IFN-β, the purified Poly5192 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated MIB-5E9.1 antibody (Cat. No. 508105) as the detection antibody and Recombinant Mouse IFN-β (ELISA Std.) (Cat. No. 581309) as the standard.

Note: For testing mouse IFN-β in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 439407 & 439408) are specially developed and recommended.

Product Citations
  1. Clua P, et al. 2017. Front Immunol. 10.3389/fimmu.2017.00948. PubMed
  2. Cheng Y, et al. 2018. J Exp Med. 215:2919. PubMed
RRID
AB_2561348 (BioLegend Cat. No. 519202)

Antigen Details

Structure
Interferon; 26-35 kD (Mammalian)
Distribution
Fibroblasts, epithelial cells
Interaction
Broad, includes NK cells, T suppressor cells, activated monocytes
Ligand/Receptor
IFNα/βR (IFNAR-1, IFNAR-2)
Cell Type
Fibroblasts, Epithelial cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U. 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
15977 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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