Recombinant Mouse LIF (carrier-free)

Pricing & Availability
Other Names
MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor), LIF (leukemia inhibitory factor)
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Product Citations
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LIF_Mouse_Recombinant_BA_071414.jpg
Mouse leukemia inhibitory factor (LIF) inhibits proliferation of M-1 cells in a dose dependent manner. The ED50 of BioLegend's mouse LIF is < 0.05 ng/ml.
  • LIF_Mouse_Recombinant_BA_071414.jpg
    Mouse leukemia inhibitory factor (LIF) inhibits proliferation of M-1 cells in a dose dependent manner. The ED50 of BioLegend's mouse LIF is < 0.05 ng/ml.
Cat # Size Price Quantity Avail. Save
554002 10 µg £82
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554004 25 µg £130
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554006 100 µg £448
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554008 500 µg £1,091
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Description

Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse LIF, amino acids (Ser24-Phe203) (Accession# NP_032527.1), was expressed in E. coli.
Molecular Mass
This 180 amino acid protein has a predicted molecular mass of approximately 20 kD. The protein migrates at approximately 20 kD in DTT-reducing conditions and approximately 18 kD in non-reducing condition by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.4.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Mouse LIF inhibits proliferation of M-1 cells with an ED50 < 0.05 ng/ml, corresponding to a specific activity of more than 20 x 106 units/mg.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Kelleher A, et al. 2017. Proc Natl Acad Sci U S A. 114(6):E1018-E1026. PubMed
  2. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  3. Jefferson WN, et al. 2020. Environ Health Perspect. 128:37001. PubMed

Antigen Details

Structure
Monomer
Distribution
Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.
Function
Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIFR
Bioactivity
mLIF inhibits proliferation of M1 cells.
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Moreau JF, et al. 1988. Nature 336:690.
2. Tomida M, et al. 1993. FEBS Lett. 334:193.
3. Metcalfe SM. 2011. Genes Immun. 12:157.
4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199.
5. Slaets H, et al. 2010. Trends Mol. Med. 16:493.

Gene ID
16878 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 09/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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