LEGEND MAX™ SARS-CoV-2 Spike RBD IgM ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
SARS-CoV-2 spike protein, 2019-nCoV, severe acute respiratory coronavirus 2 spike glycoprotein S1, SARS-CoV-2 S1 Subunit, 2019 novel coronavirus S1 protein, RBD, receptor-binding domain
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448307 1 Pre-coated Plate £291.00
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Description

SARS-CoV-2 is an enveloped non-segmented positive-sense RNA coronavirus that infects the human respiratory-epithelial cells, and is the cause of the coronavirus disease 2019 (COVID-19). One of its structural proteins includes the spike protein (S). The S protein consists of the S1 subunit which contains a receptor binding domain (RBD) that is capable of recognizing and binding with the cell surface receptor, and playing a key role in viral entry. Assays to detect virus-specific antibodies are important to understand the prevalence of infection and the course of the immune system.

The BioLegend LEGEND MAX™ SARS-CoV-2 Spike RBD Human IgM ELISA Kit is an Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with SARS-CoV-2 Spike Protein RBD. This kit is specifically designed for the accurate quantitation of SARS-CoV-2 Spike RBD Human IgM from serum and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • SARS-CoV-2 Spike RBD Human IgM Pre-coated 96-well Strip Microplate
  • SARS-CoV-2 Spike RBD Human IgM Detection Antibody
  • SARS-CoV-2 Spike RBD Human IgM lyophilized Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
SARS-CoV-2
Application

ELISA

Sensitivity
0.087 ± 0.01 pg/mL
Standard Range
0.489 - 30 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 μL to 1,000 μL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Polypropylene vials

Antigen Details

Biology Area
Adaptive Immunity, Bacterial proteins and Toxins, Immunology
Gene ID
NA
UniProt
View information about Spike RBD IgM on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 05/28/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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