- MG1-45 (See other available formats)
- Regulatory Status
- Mouse (BALB/c) IgG1, κ
- Ave. Rating
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- Product Citations
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The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.Product Details
- Antibody Type
- Host Species
- Trinitrophenol + KLH
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The immunoglobulin was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC, ICFC - Quality tested
ChIP - Verified
WB, IP, ICC, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2801452 (BioLegend Cat. No. 401401)
AB_2801451 (BioLegend Cat. No. 401402)
- Gene ID
|Purified Mouse IgG1, κ Isotype Ctrl||MG1-45||FC, ICFC, ICC, IHC, IP, WB, ChIP|
|Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl||MG1-45||FC, ICFC, WB, IP, ICC, IHC, FA|
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