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Ultra-LEAF™ Purified anti-mouse IL-3 Recombinant Antibody

Pricing & Availability
Clone
QA19A69 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A69_ULEAF_IL3_Recom_Antibody_071823
Recombinant mouse IL-3 (Cat. No. 575504) (black circles) induces the proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-mouse IL-3 recombinant (clone QA19A69) (purple squares) inhibits the effect of recombinant mouse IL-3 (Cat. No. 575504) at 1 ng/mL of M-NFS-60 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have this effect. ND50 range: 0.05-0.15 µg/mL
  • QA19A69_ULEAF_IL3_Recom_Antibody_071823
    Recombinant mouse IL-3 (Cat. No. 575504) (black circles) induces the proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-mouse IL-3 recombinant (clone QA19A69) (purple squares) inhibits the effect of recombinant mouse IL-3 (Cat. No. 575504) at 1 ng/mL of M-NFS-60 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have this effect. ND50 range: 0.05-0.15 µg/mL
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620553 100 µg 316€
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620554 1 mg 956€
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Description

IL-3 is the most potent growth factor for basophils followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant mouse IL-3
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the M-NFS-60 cell proliferation induced by recombinant mouse IL-3 (Cat. No. 575504) at 1 ng/mL. ND50 range: 0.05-0.15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068226 (BioLegend Cat. No. 620553)
AB_3068226 (BioLegend Cat. No. 620554)

Antigen Details

Structure
Cytokine
Distribution

Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells

Function
Interleukine-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures.
Interaction
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Ligand/Receptor
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Kohno M, et al. 2005. Blood.105:2059-65.
  2. Valent P and Dahinden CA. 2010. Curr Opin Hematol. 17:60-6.
  3. Kleemann R, et al. 2008. Cardiovasc Res. 79:360-76.
  4. Murphy JM and Young IG. 2006. Vitam Horm. 74:1-30.
Gene ID
16187 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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