Recombinant Human Cathepsin B (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CTSB, CPSB, APP secretase, APPS, Cathepsin B1, Epididymis Secretory Sperm Binding Protein, Amyloid Precursor Protein Secretase
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Product Citations
publications
Recom_Human-Cathepsin-B_CF_022924
Stability Testing for Recombinant Human CTSB. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
  • Recom_Human-Cathepsin-B_CF_022924
    Stability Testing for Recombinant Human CTSB. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
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557716 100 µg 240€
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Description

Cathepsin B (CTSB) is a lysosomal cysteine protease. While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. Cystatin C has been identified as an endogenous CTSB inhibitor. High CTSB protein levels and activities have been found in many tumors including breast, cervix, colon, stomach, glioma, lung, and thyroid tumors. CTSB can be secreted by tumor cells and is associated with the cell membrane of these cells. Membrane associated CTSB promotes extracellular matrix (ECM) degradation contributing to cancer motility and invasion. Many ECM proteins, including laminin, fibronectin, and collagen IV, are substrates of CTSB. CTSB can also activate pro-uPA/PLAU. Activated uPA promotes ECM digestion through serine protease plasminogen. Inhibition of CTSB can limit bone metastasis in breast cancer, making it an important anti-cancer drug target. CTSB has been proposed as a new drug target for Alzheimer's disease because of its involvement in the production of neurotoxic β-amyloid (Aβ) peptides. In a mouse model of Alzheimer’s, CTSB inhibition reduces brain Aβ peptides and improves memory. CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. Furthermore, CTSB is implicated in other pathological conditions including cardiovascular disease, multiple sclerosis, and arthritis. Roles of CTSB in autophagy, adipogenesis, and cholesterol absorption in the intestine have also been studied.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human Cathepsin B, amino acids (Arg18-Ile339) (Accession# P07858) with a N-terminal 6His tag, was expressed in E.coli
Molecular Mass
The 350 amino acid recombinant protein has a predicted molecular mass of approximately 38.89 kD. The DTT-reduced and non-reduced protein migrates at approximately 31-50 kD by SDS-PAGE.
Purity
> 85% as determined by Coomassie stained SDS-PAGE.
Formulation
Protein is formulated in 50 mM HEPES, 300 mM NaCl, 5% glycerol, pH 7.5
Concentration
100 µg at 0.5 mg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Application

Standard

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped with dry ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Cathepsin B is ubiquitous expressed and is normally localized to lysosomes. However, it can also be secreted by many cells including tumor cells, macrophages, fibroblasts, osteoclasts, T lymphocytes, and neutrophils

Function
Cathepsin B is a lysosomal cysteine protease that is normally involved in intracellular protein turnover
Interaction
Hepatitis B spliced protein, Bikunin, and TSRC1
Cell Type
Fibroblasts, Lymphocytes, Macrophages, Neutrophils, Osteoclasts, T cells
Biology Area
Immuno-Oncology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Innate Immune Signaling, Proteases
Antigen References
  1. Tang Q, et al. 2021. EMBO J. 40:e107204.
  2. Nakanishi M, et al. 2019. Cell. 177:910-924.e22.
  3. Peng T, et al. 2021. Front Immunol. 12:735643.
  4. Kumar A, et al. 2018. J Cell Sci. 131.
  5. Tong B, et al. 2014. Clinical and Experimental Immunology. 177:586-97.
Gene ID
1508 View all products for this Gene ID
UniProt
View information about Cathepsin B on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 02.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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