Purified anti-eIF-2A Antibody

Pricing & Availability
Clone
P83A7A8 (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic translation initiation factor 2A, 65 kD eukaryotic translation initiation factor 2A
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_P83A7A8_PURE_eIF-2A_Antibody_WB_082916
Total cell or tissue lysates (15 µg protein) from SH-SY5Y, HepG2, OVCAR3 and mouse brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-eIF-2A antibody (clone P83A7A8). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
  • A_P83A7A8_PURE_eIF-2A_Antibody_WB_082916
    Total cell or tissue lysates (15 µg protein) from SH-SY5Y, HepG2, OVCAR3 and mouse brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-eIF-2A antibody (clone P83A7A8). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control.
  • B_P83A7A8_PURE_eIF-2A_Antibody_WB_070618
    Total cell lysates (15 µg protein) from Daudi (lane 1), THP-1 (lane 2), HeLa (lane 3) and NIH3T3 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-eIF-2A antibody (clone P83A7A8) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-eIF-2A antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
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690302 100 µg 172,00€
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Description

eIF-2A was initially considered to be a eukaryotic functional counterpart of the prokaryotic translation initiation factor IF2, because it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner. But recent studies proved that eIF2-mediated pathway is far more efficient, and only eIF2 is able to form the 80S initiation complex on native globin mRNA. eIF2 is responsible for the loading of Met-tRNAi onto the ribosome in the majority of translation initiation events in eukaryotes, while eIF-2A is involved in the translation of a limited subset of mRNAs.

Homologues of eIF-2A are found in a wide range of eukaryotic species, indicating that eIF-2A plays some conserved roles. eIF-2A was reported to be involved in antigen presentation by major histocompatibility complex (MHC) class I molecules. Some antigenic precursors are translated from CUG codons via an unknown mechanism, which is different from the conventional methionine-initiated mechanism. Ribosome profiling experiments also revealed that translation initiation at CUG or GUG codons is a common event in yeast and human, and is not limited to antigen presentation by MHC class I molecules. Under stress conditions, such as amino acid starvation, heat shock and viral infection, eIF-2A migrates from the nucleus to the cytoplasm, where it is utilized for the translation of alphavirus 26S mRNA and hepatitis C viral (HCV) mRNA. So eIF-2A is considered to be a tRNA-loading factor in unconventional translation initiation events, such as non-AUG translation initiation, and an alternative factor of eIF2 when the active eIF2 is limited.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human eIF-2A (448-576 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the protein translated from the gene EIF2A not to be confused with the protein translated from the gene EIF2S1, often referred to as eIF2-alpha in literature, and with which it shares the common name EIF2A.

RRID
AB_2629818 (BioLegend Cat. No. 690302)

Antigen Details

Structure
585 amino acids with a predicted molecular weight of 65kD. Contains two to four WD-repeats at the N-terminus and a coiled-coil at the C-terminus.
Distribution

Cytoplasm and nucleus.

Function
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits.
Interaction
EIF2AK2, EIF3A, RIOK2, STAT1.
Biology Area
Cell Biology, Protein Synthesis
Antigen References

1. Zoll WL, et al. 2002. J. Biol. Chem. 277:37079.
2. Kashiwagi K, et al. 2014. J. Struct. Funct. Genomics. 15:125.
3. Adams SL, et al. 1975. J. Biol. Chem. 250:9083.
4. Starck SR, et al. 2012. Science. 336:1719.
5. Starck SR, et al. 2008. PLoS One. 3:e3460.
6. Ingolia NT, et al. 2009. Science. 324:218.
7. Ingolia NT, et al. 2011. Cell. 147:789.
8. Kim JH, et al. 2011. EMBO J. 30:2454.
9. Ventoso I, et al. 2006. Genes Dev. 20:87.

Gene ID
83939 View all products for this Gene ID
UniProt
View information about eIF-2alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.31.2016

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  • Purified anti-eIF-2A

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