- Regulatory Status
- Other Names
- Human Neurodegenerative Biomarker Panel 1 (5-plex), α-synuclein, Tau, Aβ42, Aβ40, NFL
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- Product Citations
Neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease represent a significant threat to human health, affecting millions of people worldwide. In order to understand the mechanism of the disease formation and its early detection, researchers need a reliable measurement tool of critical biomarkers in CSF and serum samples. The biomarkers of interest include, but are not limited to, α-Synuclein, Tau, Aβ40, Aβ42, neurofilament light chain, and various post-translationally modified and aggregated forms. The importance of these biomarkers has been extensively documented. However, questions remain on their role in disease formation and their value as diagnostic markers.
BioLegend has developed a new Multiplex assay panel to measure these five biomarkers simultaneously to meet various research needs. The Human Neurodegenerative Disease Biomarker panel is a multiplex bead-based immunoassay that utilizes fluorescence-encoded beads suitable for use on various flow cytometers. This panel provides higher detection sensitivity with broader dynamic ranges than the traditional ELISA method. It has been analytically validated and can be used to detect native proteins (α-Synuclein, Tau, Aβ40, Aβ42, and NFL) in human biological samples including serum and CSF. Please note that plasma samples are not recommended due to compromised accuracy of the assays in these samples.
Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
- Kit Contents
- Setup Beads 1: FITC Beads (1 mL)
- Setup Beads 2: PE Beads (1 mL)
- Setup Beads 3: Raw Beads (2 mL)
- Human Neurodegenerative Biomarker Panel 1 Premixed Beads (3.5 mL)
- Human Neurodegenerative Biomarker Panel 1 Detection Antibodies (3.5 mL)
- Human Neurodegenerative Biomarker Panel 1 Standard Cocktail, Lyophilized (1 vial)
- LEGENDplex™ SA-PE (3.5 mL)
- LEGENDplex™ Matrix A, Lyophilized (1 vial)
- LEGENDplex™ Assay Buffer (25 mL)
- LEGENDplex™ Wash Buffer, 20X (25 mL)
- Plate Sealers (4 sheets)
- Filter Plate
- Verified Reactivity
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.