LEGEND MAX™ Human TSLP ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
TSLP Pre-coated ELISA Kit
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434207 1 Pre-coated Plate 370€
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Description

Human TSLP (Thymic Stromal Lymphopoietin) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). TSLP is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and stromal or stromal-like cells. Human TSLP impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c+ dendritic cells. TSLP activated dendritic cells prime naïve T cells to a TH2 phenotype, which are strong producers of cytokines IL-13, IL-5, and TNF-α. TSLP also directly stimulates the production of TH2 cytokines by human mast cells. Finally, expression of TSLP has been linked to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells that trigger an inflammatory TH2 response.

BioLegend’s LEGEND MAX™ Human TSLP ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human TSLP specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human TSLP ELISA Kit is specifically designed for the accurate quantitation of Human TSLP from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human TSLP Pre-coated 96-well Strip Microplate
  • Human TSLP Dectection Antibody
  • Human TSLP Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Sensitivity
1 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs)
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
85480 View all products for this Gene ID
UniProt
View information about TSLP on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09-11-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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