Purified anti-Nuclear Pore Complex Proteins Antibody (Previously Covance catalog# MMS-120P)

Pricing & Availability
Clone
MAb414 (See other available formats)
Regulatory Status
RUO
Other Names
107 kD nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kD, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98
Previously
Covance Catalog# MMS-120P
Isotype
Mouse IgG1
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Product Citations
publications
1_MAb414_Pure_NPCP_Antibody_111417
Immunofluorescence of HeLa with Nuclear Pore Complex Proteins Mouse primary antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). Exposure time is 80 ms. Concentration is 1 µg/ml.
  • 1_MAb414_Pure_NPCP_Antibody_111417
    Immunofluorescence of HeLa with Nuclear Pore Complex Proteins Mouse primary antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). Exposure time is 80 ms. Concentration is 1 µg/ml.
  • 2_MAb414_Pure_NPCP_Antibody_ICC_122117
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Nuclear Pore Complex (Clone Mab414) overnight at 4°C followed by Alexa Fluor® 647 (Red) Goat anti-Mouse IgG (Cat. No. 405322) incubation for one hour at room temperature. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations for (A-B) is 5 µg/ml, (C) is 2 µg/ml and (D) is 0.5 µg/ml.
  • 3_MAb414_Pure_NPCP_Antibody_2_111417
    Western blot analysis of cell lysates from HaCaT, Daudi (Human), Raw264.7 (Mouse) and UMR106 (Rat) using Nuclear Pore Complex Proteins Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). GAPDH (Cat. No. 919501) was used as a loading control.
  • 4_MAb414_PURE_NPC_Antibody_052022
    IHC staining using purified anti-NPC antibody (clone Mab414) on formalin-fixed paraffin-embedded human tonsil tissue. The tissue was incubated with 1 μg/mL of anti-NPC antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mounting Solution. The image was captured with a 40x objective. Scalebar = 50 μM.
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902907 25 µL 67€
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902901 100 µL 190€
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902902 500 µL 366€
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Description

Nuclear pores are large protein complexes that cross the nuclear envelope. The proteins that make up the nuclear pore complex are known as nucleoporins. About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains. Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins). The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e. they are highly flexible proteins that lack ordered secondary structure. These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats.

Nuclear pore complexes allow the transport of molecules across the nuclear envelope. This transport includes RNA and ribosomal proteins moving from nucleus to the cytoplasm and proteins (such as DNA polymerase and lamins), carbohydrates, signaling molecules and lipids moving into the nucleus. Although smaller molecules simply diffuse through the pores, larger molecules may be recognized by specific signal sequences and then be diffused with the help of nucleoporins into or out of the nucleus.. Each of the eight protein subunits surrounding the actual pore (the outer ring) projects a spoke-shaped protein over the pore channel.

Nucleoporin p62 (p62) protein remains associated with the nuclear pore complex-lamina fraction. p62 is synthesized as a soluble cytoplasmic precursor of 61 kDa followed by modification that involve addition of N-acetylglucosamine residues, followed by association with other complex proteins.The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

P62 is a serine/threonine rich protein of ~520 amino acids, with tetrapeptide repeats on the amino terminus and a series of alpha-helical regions with hydrophobic heptad repeats. P62 assembles into a complex containing 3 addition proteins, p60, p54 and p45 forming the p62 complex of ~235 kDa. Glycosylation appears to be involved in the assembly and disassembly of p62 into higher order complexes, and a serine/threonine rich linker region between Ser270 to Thr294 appear to be regulatory. The p62 complex is localized to both the nucleoplasmic and cytoplasmic sides of the pore complex and the relative diameter of p62 complex relative to the nuclear pore complex suggests it interacts in pore gating.

Antibodies to p62 complex are involved in 1 or more autoimmune diseases. P62 glycosylation is increased in diabetes. p62 is also more frequent in Stage IV primary biliary cirrhosis and is prognostic for severe disease. Reduced p62 production has been linked to Alzheimer's disease. It is thought oxidative damage of the p62 promoter is correlated with AD and other neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Vertebrate, Xenopus, Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The antibody was raised using a nuclear pore complex mixture.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ICC - Quality tested
WB, IHC-P - Verified
IHC-F, IP, IEM - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1 μg/mL (1:1000 - 1:2000 dilution) is recommended. For Western blotting, the suggested use of this reagent is 0.2 - 0.5 µg per mL (1:2000 - 1:5000). For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a dilution of 1:500 is suggested. For immunoprecipitation and electron microscopy, the suggested dilution of this reagent is 1:5000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.

*Predicted MW = 62 kD

MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

Application References
  1. Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
  2. Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
  3. Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
  4. Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
  5. Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
  6. Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
  7. Davis LI, Blobel G. 1986. Cell. 45:699.
  8. Blobel G. 1985. PNAS USA 82:8527.
Product Citations
  1. Urbanek M, Michalak M, Krzyzosiak W 2017. Methods. 10.1016/j.ymeth.2017.04.002. PubMed
  2. Fichtman B, et al. 2019. Nat Commun. 10:605. PubMed
  3. Dilsaver MR, et al. 2018. Mol Biol Cell. :mbcE18050277. PubMed
  4. Rosso G, et al. 2019. Adv Sci (Weinh). 6:1801638. PubMed
  5. Watters K, et al. 2017. J Virol. 91:. PubMed
  6. Patil H, et al. 2019. Small GTPases. 10:146. PubMed
  7. Sołtysik K, et al. 2019. Nat Commun. 10:473. PubMed
  8. Cianciolo Cosentino C, et al. 2019. Sci Rep. 9:4750. PubMed
  9. Ahn JH, et al. 2019. Exp Mol Med. 51:64. PubMed
  10. Wild P, et al. 2019. F1000Res. 8:198. PubMed
  11. Bonnin E, et al. 2018. PLoS Genet. 14:e1007845. PubMed
  12. Behrens RT, et al. 2017. J Virol. 91. PubMed
  13. Liu Z et al. 2019. Neuron. 102(3):587-601 . PubMed
  14. Martino L et al. 2017. Developmental cell. 43(2):157-171 . PubMed
  15. Magre I, et al. 2019. J Cell Sci. 132:jcs224501. PubMed
  16. Fichtman B, et al. 2019. Am J Hum Genet. 105:48:00. PubMed
  17. Kuhn TM, et al. 2019. J Cell Biol. 218:2945. PubMed
  18. Ben-Yishay R, et al. 2019. J Cell Biol. 218:2962. PubMed
  19. Chen P, et al. 2019. J Cell Biol. 218:4063. PubMed
  20. Ohkura M 2015. Genes Dev. 29: 1789-1794. PubMed
  21. Raghunayakula S, et al. 2015. PLoS One. 10: 0144508. PubMed
  22. Zheng X, et al. 2012. J Biol Chem. 287:38254-38264. PubMed
  23. Giacomini C, et al. 2016. Mol Biol Cell. 27: 35 - 47. PubMed
  24. Berneking L, et al. 2016. PLoS Pathog. 12: 1005660. PubMed
  25. Mimura Y, et al. 2016. J Cell Sci. 129: 4200 - 4212. PubMed
  26. Alves N, et al. 2017. Sci Rep. 7:41519. PubMed
  27. Heijo H, et al. 2020. Mol Biol Cell. 31:2703. PubMed
  28. He Y, et al. 2021. eLife. 10:00. PubMed
  29. Wigington CP, et al. 2020. Molecular Cell. 79(2):342-358.e12. PubMed
  30. Duan T, et al. 2021. Current Biology. 31(7):1450-1462.e3. PubMed
RRID
AB_2734672 (BioLegend Cat. No. 902907)
AB_2565026 (BioLegend Cat. No. 902901)
AB_2565027 (BioLegend Cat. No. 902902)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References

1. Yoshimura S, et al. 2013. J. Cell Sci. 126:3141-50.

Gene ID
NA
UniProt
View information about Nuclear Pore Complex Proteins on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Nuclear Pore Complex Proteins Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Nuclear Pore Complex Proteins MAb414 ICC, WB, IHC-P, IHC-F, IP, IEM
Alexa Fluor® 594 anti-Nuclear Pore Complex Proteins MAb414 ICC
Direct-Blot™ HRP anti-Nuclear Pore Complex Proteins MAb414 WB
Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins MAb414 ICC, ICFC
TotalSeq™-A0451 anti-Nuclear Pore Complex Proteins Hashtag 1 MAb414 PG
TotalSeq™-A0452 anti-Nuclear Pore Complex Proteins Hashtag 2 MAb414 PG
TotalSeq™-A0453 anti-Nuclear Pore Complex Proteins Hashtag 3 MAb414 PG
TotalSeq™-A0454 anti-Nuclear Pore Complex Proteins Hashtag 4 MAb414 PG
TotalSeq™-A0456 anti-Nuclear Pore Complex Proteins Hashtag 6 MAb414 PG
TotalSeq™-A0457 anti-Nuclear Pore Complex Proteins Hashtag 7 MAb414 PG
TotalSeq™-A0458 anti-Nuclear Pore Complex Proteins Hashtag 8 MAb414 PG
TotalSeq™-A0459 anti-Nuclear Pore Complex Proteins Hashtag 9 MAb414 PG
TotalSeq™-A0460 anti-Nuclear Pore Complex Proteins Hashtag 10 MAb414 PG
TotalSeq™-A0455 anti-Nuclear Pore Complex Proteins Hashtag 5 MAb414 PG
TotalSeq™-A0462 anti-Nuclear Pore Complex Proteins Hashtag 12 MAb414 PG
TotalSeq™-A0463 anti-Nuclear Pore Complex Proteins Hashtag 13 MAb414 PG
TotalSeq™-A0465 anti-Nuclear Pore Complex Proteins Hashtag 15 MAb414 PG
TotalSeq™-A0461 anti-Nuclear Pore Complex Proteins Hashtag 11 MAb414 PG
TotalSeq™-A0464 anti-Nuclear Pore Complex Proteins Hashtag 14 MAb414 PG
TotalSeq™-B0453 anti-Nuclear Pore Complex Proteins Hashtag 3 MAb414 PG
TotalSeq™-B0452 anti-Nuclear Pore Complex Proteins Hashtag 2 MAb414 PG
TotalSeq™-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1 MAb414 PG
TotalSeq™-B0458 anti-Nuclear Pore Complex Proteins Hashtag 8 MAb414 PG
TotalSeq™-B0457 anti-Nuclear Pore Complex Proteins Hashtag 7 MAb414 PG
Go To Top Version: 7    Revision Date: 05-23-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Nuclear Pore Complex Proteins

  • Alexa Fluor® 594 anti-Nuclear Pore Complex Proteins

  • Direct-Blot™ HRP anti-Nuclear Pore Complex Proteins

  • Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins

  • TotalSeq™-A0451 anti-Nuclear Pore Complex Proteins Hashtag 1

  • TotalSeq™-A0452 anti-Nuclear Pore Complex Proteins Hashtag 2

  • TotalSeq™-A0453 anti-Nuclear Pore Complex Proteins Hashtag 3

  • TotalSeq™-A0454 anti-Nuclear Pore Complex Proteins Hashtag 4

  • TotalSeq™-A0456 anti-Nuclear Pore Complex Proteins Hashtag 6

  • TotalSeq™-A0457 anti-Nuclear Pore Complex Proteins Hashtag 7

  • TotalSeq™-A0458 anti-Nuclear Pore Complex Proteins Hashtag 8

  • TotalSeq™-A0459 anti-Nuclear Pore Complex Proteins Hashtag 9

  • TotalSeq™-A0460 anti-Nuclear Pore Complex Proteins Hashtag 10

  • TotalSeq™-A0455 anti-Nuclear Pore Complex Proteins Hashtag 5

  • TotalSeq™-A0462 anti-Nuclear Pore Complex Proteins Hashtag 12

  • TotalSeq™-A0463 anti-Nuclear Pore Complex Proteins Hashtag 13

  • TotalSeq™-A0465 anti-Nuclear Pore Complex Proteins Hashtag 15

  • TotalSeq™-A0461 anti-Nuclear Pore Complex Proteins Hashtag 11

  • TotalSeq™-A0464 anti-Nuclear Pore Complex Proteins Hashtag 14

  • TotalSeq™-B0453 anti-Nuclear Pore Complex Proteins Hashtag 3

  • TotalSeq™-B0452 anti-Nuclear Pore Complex Proteins Hashtag 2

  • TotalSeq™-B0451 anti-Nuclear Pore Complex Proteins Hashtag 1

  • TotalSeq™-B0458 anti-Nuclear Pore Complex Proteins Hashtag 8

  • TotalSeq™-B0457 anti-Nuclear Pore Complex Proteins Hashtag 7

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