- XMG1.2 (See other available formats)
- Regulatory Status
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- E. coli-expressed, recombinant mouse IFN-γ
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
- Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
- Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
- Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
- Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
- Ferrick D, et al. 1995. Nature 373:255. (FC)
- Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
- Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
- DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
- Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
- Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
- Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
- Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
- Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
- Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
- Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
- Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
- Product Citations
AB_961361 (BioLegend Cat. No. 505821)
AB_961359 (BioLegend Cat. No. 505822)
- Cytokine; dimer; 40-80 kD (Mammalian)
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Cell Targets
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
- Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- Gene ID
- 15978 View all products for this Gene ID
- View information about IFN-gamma on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-mouse IFN-γ
Biotin anti-mouse IFN-γ
FITC anti-mouse IFN-γ
PE anti-mouse IFN-γ
Purified anti-mouse IFN-γ
Alexa Fluor® 488 anti-mouse IFN-γ
Alexa Fluor® 647 anti-mouse IFN-γ
Pacific Blue™ anti-mouse IFN-γ
PerCP/Cyanine5.5 anti-mouse IFN-γ
PE/Cyanine7 anti-mouse IFN-γ
Brilliant Violet 421™ anti-mouse IFN-γ
Brilliant Violet 650™ anti-mouse IFN-γ
Ultra-LEAF™ Purified anti-mouse IFN-γ
Brilliant Violet 711™ anti-mouse IFN-γ
Brilliant Violet 785™ anti-mouse IFN-γ
Brilliant Violet 605™ anti-mouse IFN-γ
Brilliant Violet 510™ anti-mouse IFN-γ
Purified anti-mouse IFN-γ (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse IFN-γ
Alexa Fluor® 700 anti-mouse IFN-γ
APC/Cyanine7 anti-mouse IFN-γ
GoInVivo™ Purified anti-mouse IFN-γ
APC/Fire™ 750 anti-mouse IFN-γ
Spark NIR™ 685 anti-mouse IFN-γ