PE/Dazzle™ 594 anti-mouse CD154 (CD40L) Antibody

Pricing & Availability
Clone
SA047C3 (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5, CD40 Ligand
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA047C3_PEDazzel594_CD154-_Antibody_032922
Enriched BALB/c mouse splenic T cells were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No.423301) for 6 hours and stained with anti-mouse CD154 (CD40L) (clone SA047C3) PE/Dazzle 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
  • SA047C3_PEDazzel594_CD154-_Antibody_032922
    Enriched BALB/c mouse splenic T cells were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No.423301) for 6 hours and stained with anti-mouse CD154 (CD40L) (clone SA047C3) PE/Dazzle 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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157015 25 µg 93€
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Description

CD154 has been kown a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation and Ig class switching.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCD40L-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2922488 (BioLegend Cat. No. 157015)

Antigen Details

Structure
A 39 kD TNF superfamily memeber
Distribution

Surface activated CD4+ T cells,B cells, macrophages, dendritic cells, mast cells, NK cells, polymorphs, monocytes, and activated platelets.

Function
Survival signaling to CD40+ B cells and Ig class switching.
Ligand/Receptor
CD40
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03-29-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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